INTERLEUKIN-1 UP-REGULATES DECORIN PRODUCTION BY ARTERIAL SMOOTH-MUSCLE CELLS

被引：36

作者：

EDWARDS, IJ

XU, HZ

WRIGHT, MJ

WAGNER, WD

机构：

[1] Department of Comparative Medicine, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC

[2] Department of Comparative Medicine, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157-1040, Medical Center Blvd

来源：

ARTERIOSCLEROSIS AND THROMBOSIS | 1994年 / 14卷 / 07期

关键词：

INTERLEUKIN-1; PROTEOGLYCANS; SMOOTH MUSCLE CELLS;

D O I：

10.1161/01.ATV.14.7.1032

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

An increase in dermatan sulfate-proteoglycan (DSPG) production occurs in cultured aortic smooth muscle cells exposed to macrophage-conditioned media, an effect that is abrogated by an antibody to interleukin-1 (IL-1). To determine which DSPG gene was regulated, cultured arterial smooth muscle cells from monkeys (Macaca fascicularis) were treated with 0 to 500 pg/mL human recombinant IL-1 alpha or IL-1 beta in the presence of [S-35]sulfate and [H-3]serine. Proteoglycans were isolated from the culture media and purified by selective precipitation and chromatography. Both recombinant IL-1 alpha and IL-1 beta caused a dose-response increase in DSPG production. Northern blot analysis of mRNA isolated from the cells identified 1.6-kb and 2.6-kb

引用

页码：1032 / 1039

页数：8

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