HPLC-CL联用在线检测消癌平注射液清除HO及O-自由基活性

被引:7
作者
朱卉
朱丹妮
余伯阳
机构
[1] 中国药科大学中药复方教研室
关键词
消癌平; HPLC-CL; 绿原酸; 自由基;
D O I
暂无
中图分类号
R284.1 [化学分析与鉴定];
学科分类号
摘要
目的:利用HPLC-CL在线检测消癌平注射液(通关藤)对H2O2和O2·-自由基的清除活性,追踪其物质基础。方法:消癌平注射经HPLC后进入T型混合管(内含鲁米诺+过氧化氢溶液和鲁米诺+连苯三酚),记录化学发光信号结合LC-MS/MS鉴定其结构。结果:已鉴定出3-咖啡酰奎尼酸、5-咖啡酰奎尼酸和4-咖啡酰奎尼酸3种物质,清除自由基能力较强。结论:消癌平注射液中酚酸类成分对其清除H2O2和O2·-自由基有很大贡献;HPLC-CL在线联用技术简单、快速,可用于中药抗氧化活性成分的筛选。
引用
收藏
页码:1386 / 1390
页数:5
相关论文
共 16 条
[11]  
Determination of nine pyrethroid insecticides by high-performance liquid chromatography with post-column photoderivatization and detection based on acetonitrile chemiluminescence[J] M. Martínez Galera;M.D. Gil García;R. Santiago Valverde Journal of Chromatography A 2006,
[12]  
Sensitive determination of phenolic compounds using high-performance liquid chromatography with cerium(IV)-rhodamine 6G-phenolic compound chemiluminescence detection[J] Qunlin Zhang;Hua Cui;Aung Myint;Mei Lian;Lijuan Liu Journal of Chromatography A 2005,
[13]  
Neuroprotective effects of anesthetic agents[J] Masahiko Kawaguchi;Hitoshi Furuya;Piyush M. Patel Journal of Anesthesia 2005,
[14]  
Enhancing and inhibiting effects of aromatic compounds on luminol–dimethylsulfoxide–OH ? chemiluminescence and determination of intermediates in oxidative hair dyes by HPLC with chemiluminescence detection[J] Jian Zhou;Hong Xu;Guo-Hui Wan;Chun-Feng Duan;Hua Cui Talanta 2004,
[15]  
Determination of polyphenols by high-performance liquid chromatography with inhibited chemiluminescence detection[J] Hua Cui;Caixia He;Guiwen Zhao Journal of Chromatography A 1999,
[16]  
Role of oxygen free radicals in cancer development[J] D. Dreher;A.F. Junod European Journal of Cancer 1996,