实时荧光定量PCR技术在白酒酿造过程中微生物数量检测的应用展望附视频

被引:4
作者
程平言
路虎
胡峰
涂华彬
机构
[1] 贵州茅台酒厂(集团)习酒有限责任公司
关键词
白酒酿造; 荧光定量; PCR; 应用;
D O I
10.13746/j.njkj.2020026
中图分类号
TS262.3 [白酒]; O657.3 [光化学分析法(光谱分析法)];
学科分类号
摘要
实时荧光定量PCR (real-time fluorescent quantitative polymerase chain reaction)是将普通PCR和光谱分析、实时检测等手段巧妙结合应用的一项技术。具有特异性强、灵敏度高、重复性好、定量准确、自动化程度高等优点,已成为分子生物学领域的重要技术工具。尤其是在微生物检测方面得到极大的推广应用。白酒酿造过程中微生物种类复杂、数量在不断变化,可培养方法的局限性很大,但是采用荧光定量PCR的方法有较理想的效果。本文对荧光定量PCR的原理、扩增序列的选择、基因组提取方法等方面做一综述。
引用
收藏
页码:74 / 77
页数:4
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