限制性内切酶在细胞遗传学中的应用

被引：2

作者：

董伟峰

张思仲

机构：

[1] 华西医科大学医学遗传学研究室

来源：

遗传与疾病 | 1986年 / 04期

关键词：

限制酶; DNA; 序列; 染色体显带; 核酸酶; 异染色质; 染色体畸变率; 内切酶; 细胞遗传学; 限制性;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

<正> 限制性内切酶(restriction endonuclease,简称限制酶)是一类能识别并切开特定DNA序列的核酸内切酶。已发现的限制酶将近500种,其中50余种已广泛应用。限制酶分为三型:Ⅰ型酶由不同的亚基组成,具有ATP依赖性的水解活性和甲基化活性。Ⅱ型酶由相同亚基构成,仅具限制酶活性,它们能识别的DNA专一性序列通常为4～6个碱基对,并在特定位点将底物水解,因而在分子遗传学中广泛应用。Ⅲ型酶仅有数种,如HinfⅢ、EcoPⅠ等,但其切点未定。

引用

页码：236 / 239

页数：4

共 10 条

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