ACC合成酶基因及其反义基因对西瓜的遗传转化

被引：37

作者：

王春霞

简志英

刘愚

邹琦

白永延

毛慧珠

机构：

[1] 山东农业大学,中国科学院上海植物生理研究所,山东农业大学,中国科学院上海植物生理研究所,中国科学院上海植物生理研究所泰安,上海,泰安,上海,上海

来源：

Acta Botanica Sinica | 1997年 / 05期

关键词：

转基因西瓜; 根癌农杆菌; ACC合成酶基因; 反义基因;

D O I：

暂无

中图分类号：

S651 [西瓜];

学科分类号：

摘要：

以2日龄西瓜(Citrullus lanatus（Thunb.）Mansfeld)无菌苗子叶为外植体,通过与根癌农杆菌（Agrobacterium tumefaciens）进行叶盘共培养建立了西瓜的遗传转化系统。所用根癌农杆菌中含有改建后分别携带嵌合NPTⅡ基因和番茄的ACC合成酶基因及其反义基因的质粒。外植体在MSA培养基（MS盐类、B5维生素、1.0mg/L BA、0.2mg/L IAA）上预培养3～4d后,与根癌农杆菌共培养4d,随后转移外植体至附加100mg/L卡那霉素、300mg/L头孢菌素的MSA培养基上筛选转化芽。将带芽外植体移入含有100mg/L卡那霉素、300mg/L头孢菌素的伸长培养基（MS+0.2mg/L KT）上进行芽伸长,切取2～3cm高的伸长芽移入生根培养基（1/2MS+0.1mg/L NAA）生根。Southern blot结果证明获得转基因植株,乙烯释放指标表明转入的正义和反义ACC合成酶基因得到不同程度的表达。

引用

页码：445 / 450

页数：6

共 13 条

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