叶绿体SSR标记:柑橘体细胞杂种胞质遗传分析的一种新方法(英文)

被引:16
作者
程运江
郭文武
邓秀新
机构
[1] 华中农业大学作物遗传改良国家重点实验室
[2] 华中农业大学作物遗传改良国家重点实验室 武汉
[3] 武汉
关键词
柑橘; 体细胞杂种; 微卫星序列:; 简单序列重复(SSR); 叶绿体基因组;
D O I
暂无
中图分类号
S666 [柑桔类];
学科分类号
090201 ;
摘要
从水稻(Oryza sativa L.)、烟草(Nicotiana tabacum L.)和黑松(Pinus thunbergii Parl.)等植物的22对叶绿体SSR引物中筛选出5对能用于柑橘叶绿体SSR分析的引物,应用这5对引物对9个组合的柑橘体细胞杂种的叶绿体遗传进行了分析。结果表明:这些组合再生的杂种中叶绿体都呈现随机分离,该现象与以前报道的RFLP分析结果一致,而且其可靠性已被CAPS分析所证实。表明柑橘叶绿体SSR同RFLP及CAPS一样可靠,并且更简单高效、易于操作,特别适合对柑橘等植物体细胞杂种进行早期胞质遗传组成分析。
引用
收藏
页码:906 / 909
页数:4
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