影响甘蓝下胚轴原生质体转化的因素

被引：5

作者：

薛红卫

卫志明

许智宏

机构：

[1] 中国科学院上海植物生理研究所!上海

来源：

植物生理学通讯 | 1994年 / 04期

关键词：

甘蓝的下胚轴; 原生质体; 转化;

D O I：

暂无

中图分类号：

Q943 [植物细胞遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

PEG法介导甘蓝原生质体转化时以在转化后48h瞬间表达强度最高；当转化介质为15mmol／L的Ca2+离子，加入CT－DNA、质粒DNA各50μg，PEG终浓度为12％，处理30min，转化后采用逐步稀释法时，转化效率最高。

引用

页码：249 / 255

页数：7

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