Pseudomonas aeruginosa Type III secretion system interacts with phagocytes to modulate systemic infection of zebrafish embryos

被引:100
作者
Brannon, Mark K. [8 ]
Davis, J. Muse [2 ]
Mathias, Jonathan R. [3 ]
Hall, Chris J. [4 ]
Emerson, Julia C. [8 ]
Crosier, Philip S. [4 ]
Huttenlocher, Anna [3 ,5 ]
Ramakrishnan, Lalita [1 ,6 ,7 ]
Moskowitz, Samuel M. [8 ]
机构
[1] Univ Washington, Dept Microbiol, Seattle, WA 98195 USA
[2] Emory Univ, Immunol & Mol Pathogenesis Program, Atlanta, GA 30322 USA
[3] Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA
[4] Univ Auckland, Sch Med Sci, Dept Mol Med & Pathol, Auckland 1, New Zealand
[5] Univ Wisconsin, Dept Pediat, Madison, WI USA
[6] Univ Washington, Dept Med, Seattle, WA USA
[7] Univ Washington, Dept Immunol, Seattle, WA 98195 USA
[8] Univ Washington, Dept Pediat, Seattle, WA 98195 USA
基金
美国国家卫生研究院;
关键词
STAPHYLOCOCCUS-AUREUS INFECTION; INNATE IMMUNE-RESPONSE; CAENORHABDITIS-ELEGANS; EXOENZYME-S; MODEL HOST; PROTEIN SECRETION; EPITHELIAL-CELLS; RHO GTPASES; MACROPHAGES; DROSOPHILA;
D O I
10.1111/j.1462-5822.2009.01288.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Pseudomonas aeruginosa is an opportunistic human pathogen that can cause serious infection in those with deficient or impaired phagocytes. We have developed the optically transparent and genetically tractable zebrafish embryo as a model for systemic P. aeruginosa infection. Despite lacking adaptive immunity at this developmental stage, zebrafish embryos were highly resistant to P. aeruginosa infection, but as in humans, phagocyte depletion dramatically increased their susceptibility. The virulence of an attenuated P. aeruginosa strain lacking a functional Type III secretion system was restored upon phagocyte depletion, suggesting that this system influences virulence through its effects on phagocytes. Intravital imaging revealed bacterial interactions with multiple blood cell types. Neutrophils and macrophages rapidly phagocytosed and killed P. aeruginosa, suggesting that both cell types play a role in protection against infection. Intravascular aggregation of erythrocytes and other blood cells with resultant circulatory blockage was observed immediately upon infection, which may be relevant to the pathogenesis of thrombotic complications of human P. aeruginosa infections. The real-time visualization capabilities and genetic tractability of the zebrafish infection model should enable elucidation of molecular and cellular details of P. aeruginosa pathogenesis in conditions associated with neutropenia or impaired phagocyte function.
引用
收藏
页码:755 / 768
页数:14
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