Immunomodulatory effects of holothurian triterpene glycosides on mammalian splenocytes determined by mass spectrometric proteome analysis

被引：37

作者：

Aminin, Dmitri L. ^{[2
]}

Koy, Conelia ^{[1
]}

Dmitrenok, Pavel S. ^{[2
]}

Mueller-Hilke, Brigitte ^{[3
]}

Koczan, Dirk ^{[3
]}

Arbogast, Brian ^{[4
]}

Silchenko, Aleksandra A. ^{[2
]}

Kalinin, Vladimir I. ^{[2
]}

Avilov, Sergey A. ^{[2
]}

Stonik, Valentin A. ^{[2
]}

Collin, Peter D. ^{[5
]}

Thiesen, Hans-Juergen ^{[3
]}

Deinzer, Max L. ^{[4
]}

Glocker, Michael O. ^{[1
]}

机构：

[1] Univ Rostock, Proteome Ctr Rostock, D-18057 Rostock, Germany

[2] Russian Acad Sci, Pacific Inst Bioorgan Chem, Far E Div, Vladivostok 690022, Russia

[3] Univ Rostock, Inst Immunol, D-18057 Rostock, Germany

[4] Oregon State Univ, Dept Chem, Corvallis, OR 97331 USA

[5] Coastside Bioresources, Stonington, ME 04681 USA

来源：

JOURNAL OF PROTEOMICS | 2009年 / 72卷 / 05期

关键词：

Triterpene glycosides; Immuno-stimulation; Proteome analysis; Mass spectrometry; Cell proliferation; Cell adhesion; LYMPHOCYTE-PROLIFERATION; DEPENDENT CHANGES; FRONDOSIDE-A; EXPRESSION; PROTEINS; ADAPTER; DIFFERENTIATION; IDENTIFICATION; SEPTINS;

D O I：

10.1016/j.jprot.2009.04.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Spleen is a prime organ in which immuno-stimulation takes place in mammalians. Proteome analysis was used to investigate the elicited effects on mouse splenocytes upon exposure to holothurian triterpene glycosides. Cucumarioside A(2)-2, and Frondoside A, respectively, have been used to in-vitro stimulate primary splenocyte cultures. Differential protein expression was monitored by 2D gel analysis and proteins in spots of interest were identified by MALDI ToF MS and nano LC-ESI Q-ToF MS/MS, respectively. Differential image analysis of gels from control us. gels from stimulated primary splenocyte cultures showed that approximately thirty protein spots were differentially expressed. Prime examples of differentially expressed proteins are NSFL1 cofactor p47 and hnRNP K (down-regulated), as well as Septin-2, NADH dehydrogenase [ubiquinone) iron-sulfur protein 3, and GRB2-related adaptor protein 2 (up-regulated). Immuno-analytical assays confirmed differential protein expression. Together with results from proliferation and cell adhesion assays, our results show that cellular proliferation is stimulated by holothurian triterpene glycosides. In conclusion, holothurian triterpene glycosides are thought to express their immunostimulatory effects by enhancing the natural cellular defense barrier that is necessary to fight pathogens and for which lymphocytes and splenocytes have to be recruited constantly due to limited lifetimes of B-cells and T-cells in the body. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：886 / 906

页数：21

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