哈茨木霉CGMCC 2979生物转化栀子中的京尼平苷制备京尼平

被引：16

作者：

董悦生 ^{[1
]}

刘乐平 ^{[1
]}

包永明 ^{[1
]}

郝爱鱼 ^{[2
]}

秦莹 ^{[1
]}

温祖佳 ^{[1
]}

修志龙 ^{[1
]}

机构：

[1] 大连理工大学生命科学与技术学院

[2] 大连市药品检验所

来源：

催化学报 | 2014年 / 35卷 / 09期

关键词：

京尼平苷; 京尼平; 哈茨木霉; 生物转化; 京尼平苷β-葡萄糖苷酶; 纯化.;

D O I：

暂无

中图分类号：

TQ461 [中草药制剂的生产];

学科分类号：

091003 [林草生态工程学];

摘要：

采用微生物直接转化药材的方法,将栀子中的京尼平苷转化为京尼平,无需糖苷酶和京尼平苷的制备.在培养温度为30°C,pH 6.1以及栀子载量为80 g/L的条件下,48 h京尼平苷的转化率为97.8%.转化后的京尼平通过XAD-16N大孔树脂偶联硅胶层析的方法,制备得到纯度大于95%的京尼平,收率为62.3%.在催化、转化机制研究中,从哈茨木霉CGMCC2979的发酵液中分离得到了分子量为74.4 k Da的京尼平苷β-葡萄糖苷酶,该酶最优催化条件为50°C和pH 4.0–5.0.K m和V max分别为3.6 mmol/L和775μmol/h/mg蛋白.本文提供了一种简便、高效制备京尼平的新方法.

引用

页码：1534 / 1546

页数：13

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