Novel biocatalysts for white biotechnology

被引:43
作者
Institute of Molecular Enzyme Technology, Heinrich-Heine-University Düsseldorf, Research Center Jülich, Jülich, Germany [1 ]
机构
[1] Institute of Molecular Enzyme Technology, Heinrich-Heine-University Düsseldorf, Research Center Jülich, Jülich
来源
Biotechnol. J. | 2006年 / 7-8卷 / 777-786期
关键词
Biocatalysis; Directed evolution; Metagenomics; Overexpression;
D O I
10.1002/biot.200600059
中图分类号
学科分类号
摘要
White Biotechnology uses microorganisms and enzymes to manufacture a large variety of chemical products. Therefore, the demand for new and useful biocatalysts is steadily and rapidly increasing. We have developed methods for the isolation of new enzyme genes, constructed novel expression systems, and optimized existing enzymes for biotechnological applications by methods of directed evolution. Furthermore, we have isolated and characterized biocatalysts relevant for the preparation of enantiopure compounds. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码:777 / 786
页数:9
相关论文
共 33 条
[31]  
Hummel W., Reduction of acetophenone to R(+)-phenylethanol by a new alcohol dehydrogenase from Lactobacillus kefir, Appl. Microbiol. Biotechnol., 34, pp. 15-19, (1990)
[32]  
Bradshaw C.W., Hummel W., Wong C.-H., Lactobacillus kefir alcohol dehydrogenase: a useful catalyst for synthesis, J. Org. Chem., 57, pp. 1532-1536, (1992)
[33]  
Weckbecker A., Hummel W., Improved synthesis of chiral alcohols with Escherichia coli cells co-expressing pyridine nucleotide transhydrogenase NADP+-dependent alcohol dehydrogenase and NAD <sup>+</sup>-dependent formate dehydrogenase, Biotechnol. Lett., 26, pp. 1739-1744, (2004)