Micro-MRI at 11.7 T of a Murine Brain Tumor Model Using Delayed Contrast Enhancement

被引：13

作者：

Moats, Rex A. ^{[1
,2
,3
]}

Velan-Mullan, Sendhil ^{[2
]}

Jacobs, Russell ^{[2
]}

Gonzalez-Gomez, Ignacio ^{[1
]}

Dubowitz, David J. ^{[1
]}

Taga, Takashi ^{[1
]}

Khankaldyyan, Vazgen ^{[1
]}

Schultz, Linda ^{[1
]}

Fraser, Scott ^{[2
]}

Nelson, Marvin D. ^{[1
]}

Laug, Walter E. ^{[1
]}

机构：

[1] USC Keck School of Medicine, Los Angeles, CA

[2] California Institute of Technology, Pasadena, CA

[3] Department of Radiology, Childrens Hospital Los Angeles, MS 81, Los Angeles, CA 90027

来源：

Molecular Imaging | 2003年 / 2卷 / 03期

关键词：

Magnetic resonance imaging - Medical imaging - Pathology - Tumors;

D O I：

10.1162/153535003322556895

中图分类号：

学科分类号：

摘要：

In vivo imaging methodologies allow for serial measurement of tumor size, circumventing the need for sacrificing mice at given time points. In orthotopically transplanted murine models of brain tumors, cross-section micro-MRI allows for visualization and measurement of the physically inaccessible tumors. To allow for long resident times of a contrast agent in the tumor, intraperitoneal administration was used as a route of injection for contrast-enhanced micro-MRI, and a simple method for relative tumor volume measurements was examined. A strategy for visualizing the variability of the delayed tumor enhancement was developed. These strategies were applied to monitor the growth of brain tumors xenotransplanted into nude mice and either treated with the antiangiogenic peptide EMD 121974 or an inactive control peptide. Each mouse was used as its own control. Serial imaging was done weekly, beginning at Day 7 after tumor cell implantation and continued for 7 weeks. Images obtained were reconstructed on the MKI instrument. The image files were transferred off line to be postprocessed to assess tumor growth (volume) and variability in enhancement (three-dimensional [3-D] intensity models). In a small study, tumor growth and response to treatment were followed using this methodology and the high-resolution images displayed in 3-D allowed for straightforward qualitative assessment of variable enhancement related to vascular factors and tumor age.

引用

页码：150 / 158

页数：8

共 33 条

[11]

Dong Z., Radinsky R., Fan D., Tsan R., Bucana C.D., Wilmanns C., Fidler I.J., Organ-specific modulation of steady-state mdr gene expression and drug resistance in murine colon cancer cells, J Natl Cancer Inst, 86, pp. 913-920, (1994)

[12]

MacDonald T.J., Taga T., Shimada H., Tabrizi P., Zlokovic B.V., Cheresh D.A., Laug W.E., Preferential susceptibility of brain tumors to the antiangiogenic effects of an alpha (v) integrin antagonist, Neurosurgery, 48, pp. 151-157, (2001)

[13]

Fujimori H., Matsumura A., Yamamoto T., Shibata Y., Yoshizawa T., Nakagawa K., Yoshii Y., Nose T., Sakata I., Nakajima S., Tumor specific contrast enhancement study of Mn-metalloporphyrin (ATN-10) - Comparison of rat brain tumor model, cytotoxic and vasogenic edema models, Acta Neurochir Suppl (Wien), 70, pp. 167-169, (1997)

[14]

Vohra S., Rent G., Campbell V., Abbott M., Whyte R., Effect of polyethlene occlusive skin wrapping on heat loss in very low birth weight infants at delivery: A randomized trial, J Pediatr, 134, pp. 547-551, (1999)

[15]

Jacobs R.E., Fraser S.E., Imaging neuronal development with magnetic resonance Imaging (NMR) microscopy, Journal of Neuroscience Methods, 54, pp. 189-196, (1994)

[16]

Folkman J., Anti-angiogenesis: New concept for therapy of solid tumors, Ann Surg, 175, pp. 409-416, (1972)

[17]

Zagzag D., Amirnovin R., Greco M.A., Yee H., Holash J., Wiegand S.J., Zabski S., Yancopoulos G.D., Grumet M., Vascular apoptosis and involution in gliomas precede neovascularization: A novel concept for glioma growth and angiogenesis, Lab Invest, 80, pp. 837-849, (2000)

[18]

Holash J., Maisonpierre P.C., Compton D., Boland P., Alexander C.R., Zagzag D., Yancopoulos G.D., Wiegand S.J., Vessel cooption, regression, and growth in tumors mediated by angiopoietins and VEGF, Science, 284, pp. 1994-1998, (1999)

[19]

Daldrup H., Shames D.M., Wendland M., Okuhata Y., Link T.M., Rosenau W., Lu Y., Brasch R.C., Correlation of dynamic contrast-enhanced MR imaging with histologic tumor grade: Comparison of macromolecular and small-molecular contrast media, AJR Am J Roentgenol, 171, pp. 941-949, (1998)

[20]

Weissleder R., Bogdanov Jr. A., Tung C.H., Weinmann H.J., Size optimization of synthetic graft copolymers for in vivo angiogenesis imaging, Bioconjug Chem, 12, pp. 213-219, (2001)

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