3' TERMINAL SEQUENCES OF HUMAN ALPHA AND BETA GLOBIN MESSENGER-RNAS - COMPARISON WITH RABBIT GLOBIN MESSENGER-RNA

被引：50

作者：

PROUDFOOT, NJ

LONGLEY, JI

机构：

[1] MRC, MOLEC BIOL LAB, CAMBRIDGE CB2 2QH, ENGLAND

[2] RADCLIFFE INFIRM, NUFFIELD DEPT CLIN MED, OXFORD OX2 6HE, ENGLAND

来源：

CELL | 1976年 / 9卷 / 04期

关键词：

D O I：

10.1016/0092-8674(76)90137-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sequences of 43 and 70 nucleotides adjacent to the 3'' terminal poly(A) of human .alpha. and .beta. globin mRNAs were established. Sequence analysis of complementary DNA from both normal and thalassemic globin mRNA preparations was carried out using endonuclease IV digestion and limited synthesis procedures. The 2 RNA sequences are 83% homologous to rabbit globin mRNA, and a part of the .alpha. globin mRNA sequence codes for the carboxy terminus of human .alpha. globin Constant Spring. This latter result predicts that the 3'' noncoding region of human .alpha. globin mRNA is exactly 112 nucleotides, while the 5'' noncoding region is probably about 50 nucleotides.

引用

页码：733 / 746

页数：14

共 32 条

[1] CHROMATOGRAPHY OF P-32-LABELLED OLIGONUCLEOTIDES ON THIN LAYERS OF DEAE-CELLULOSE [J].