EMA-LAMP方法快速检测死/活的食源性沙门氏菌

被引：18

作者：

鲁玉侠

郭祀远

石磊

李琳

机构：

[1] 华南理工大学轻工与食品学院

来源：

食品科学 | 2009年 / 30卷 / 22期

关键词：

沙门氏菌; 快速检测; EMA-LAMP;

D O I：

暂无

中图分类号：

TS207.4 [食品的微生物检验];

学科分类号：

083201 ;

摘要：

建立应用叠氮溴化乙锭(ethidium monoazide,EMA)与环介导等温扩增(loop-mediated isothermal amplification,LAMP)相结合检测沙门氏死/活菌的方法。该方法具有快速、方便和灵敏度高等优点,以SYBRGreenI为显色剂、恒温65℃、反应1h便可得出检测结果。该检测系统中,得到241bp的目标片段和类似于梯子状的系列条带。该方法的检测限为10-10gDNA/管,是EMA-PCR方法的1%。本方法实用性强,具有普遍的应用意义。

引用

页码：324 / 327

页数：4

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