Notch1信号通路对胃癌细胞增殖的影响

被引：7

作者：

默娟

李红

机构：

[1] 辽宁医学院生物化学教研室

来源：

山东医药 | 2012年 / 52卷 / 07期

关键词：

胃癌细胞; Notch1; 激活剂; 抑制剂; 细胞增殖; 细胞周期;

D O I：

暂无

中图分类号：

R735.2 [胃肿瘤];

学科分类号：

100214 ;

摘要：

目的探讨Notch1信号通路对体外培养的胃癌细胞(BGC-823)增殖的影响,为胃癌的治疗提供新思路。方法将体外培养的胃癌细胞株BGC-823分为三组,空白组加入PBS缓冲液,激活剂组加入Notch1通路激活剂rh-NF-κB,抑制剂组加入Notch1通路抑制剂γ-secretase inhibitorⅡ。Western blot法检测各组Notch-1和Hes-1蛋白表达;MTT法检测细胞增殖情况;流式细胞仪检测细胞周期分布。结果与空白组比较,激活剂组Notch-1和Hes-1呈高表达、抑制剂组呈低表达;激活剂组细胞增殖减低,抑制剂组增殖旺盛;激活剂组S期胃癌细胞所占比例低,抑制剂组所占比例高。P均<0.05。结论 Notch1信号通路激活能抑制胃癌细胞增殖。

引用

页码：15 / 17

页数：3

共 14 条

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