HPLC-MS/MS同时检测人尿中巴比妥、苯巴比妥、异戊巴比妥、司可巴比妥

被引：8

作者：

朱鹏飞

刘文卫

周闰

凌霞

机构：

[1] 江苏省无锡市疾病预防控制中心

来源：

中国卫生检验杂志 | 2012年 / 22卷 / 09期

关键词：

巴比妥; 苯巴比妥; 异戊巴比妥; 司可巴比妥; 尿液; 液相色谱-串联质谱联用法;

D O I：

暂无

中图分类号：

R927 [药物鉴定]; O657.63 [质谱分析];

学科分类号：

1007 ; 070302 ; 081704 ;

摘要：

目的:建立测定尿液中巴比妥类药物水平[巴比妥(Barbital,bar)、苯巴比妥(Phenobarbital,phe)、异戊巴比妥(Amobarbital,amo)、司可巴比妥(Secobarbital,sec)]的高灵敏度的高效液相色谱-串联质谱联用(HPLC-MS/MS)分析方法。方法:尿液经乙醚+环己烷(1∶1)液液萃取、氮气吹干浓缩后,用高效液相色谱-串联质谱联用仪分析。结果:尿液中4种巴比妥类药物在0.01 mg/L～0.2 mg/L浓度范围内呈良好线性,线性相关系数r均大于0.99;加标回收率为91.3%～106.8%;bar的方法定量检出限为0.005 mg/L;amo、sec、phe的方法定量检出限为0.002 mg/L。结论:该方法检出限较低、线性范围宽、分析时间较短,可用于检测人体尿液中巴比妥类药物的含量。

引用

页码：2003 / 2006

页数：4

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