rTMS促进大鼠局灶性脑缺血海马内源性神经干细胞分化的研究

被引:9
作者
尹清
刘宏亮
汪琴
余洪俊
颜如冰
机构
[1] 第三军医大学西南医院康复理疗科
关键词
重复经颅磁刺激; 局灶性脑缺血; 海马; 神经干细胞; 分化;
D O I
暂无
中图分类号
R743.3 [急性脑血管疾病(中风)];
学科分类号
1002 ;
摘要
目的探讨重复经颅磁刺激(rTMS)对局灶性脑缺血大鼠海马内源性神经干细胞分化的影响。方法线栓法制备大鼠大脑中动脉闭塞(MCAO)模型,随机分为脑缺血自然恢复组和rTMS治疗组,用荧光显微镜和共聚焦显微镜观察缺血14d、28d后各组大鼠海马中5-溴脱氧尿核苷(BrdU)与神经元特异核蛋白(NeuN)、神经胶质酸性蛋白(GFAP)共同标记的阳性细胞,并在高倍荧光显微镜下对双标阳性细胞计数。结果脑缺血后14d、28d,rTMS治疗组大鼠海马BrdU/NeuN双标阳性细胞数量分别为17.12±2.91、23.20±5.97,较相应自然恢复组12.96±2.79、15.92±2.52明显增加,两组同一时间点组间比较有统计学差异(P<0.01)。而脑缺血后14d、28d,rTMS治疗组大鼠海马BrdU/GFAP双标阳性细胞数量分别为30.48±4.58、36.48±4.90,较相应自然恢复组37.44±3.58、43.60±5.96减少,两者相比有统计学差异(P<0.05)。结论局灶性脑缺血大鼠海马增殖的内源性神经干细胞,可分化为神经元或神经胶质细胞,而rTMS可促进海马内源性神经干细胞向神经元的分化。
引用
收藏
页码:49 / 53
页数:5
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