基于全病毒颗粒的中东呼吸综合征冠状病毒抗体检测方法的建立

被引：2

作者：

王慧娟 ^{[1
]}

王文玲 ^{[1
]}

蓝佳明 ^{[1
]}

宋铁 ^{[2
]}

甄维 ^{[1
]}

周为民 ^{[1
]}

柯昌文 ^{[2
]}

武桂珍 ^{[1
]}

谭文杰 ^{[1
]}

机构：

[1] 北京, 中国疾病预防控制中心病毒病预防控制所, 卫生和计划生育委员会医学病毒学与病毒病重点实验室

[2] 广州,

来源：

中华微生物学和免疫学杂志 | 2016年 / 36卷 / 02期

关键词：

中东呼吸综合征; 冠状病毒; 抗体; 酶联免疫吸附试验;

D O I：

暂无

中图分类号：

R392 [医学免疫学]; R446.6 [免疫学检验];

学科分类号：

100102 ;

摘要：

目的建立基于全病毒颗粒的中东呼吸综合征冠状病毒(Middle East respiratory syndrome coronavirus, MERS-CoV)感染的抗体检测方法, 并将该方法用于MERS相关病例血清抗体(IgG与IgM)的检测。方法采用基于灭活MERS-CoV颗粒作为检测用靶抗原, 建立酶联免疫检测方法(ELISA), 选取10份新生儿血清和40份正常成年人血清标本, 确定MERS-CoV IgM和IgG抗体检测界值;在此基础上对我国第1例MERS感染病例的5份系列血清标本抗MERS-CoV抗体进行检测。结果全病毒灭活颗粒抗原与正常成人或儿童血清不存在明显交叉反应, 可确定MERS-CoV抗体IgM和IgG检测界值A450分别为0.32和0.42。我国输入性MERS病例入院初期血清IgM和IgG抗体滴度均为1︰40, 入院2周后IgM和IgG抗体阳转, 滴度均有4倍以上升高, 入院4周后IgG滴度可达到1︰640。结论建立了基于灭活全病毒颗粒的MERS-CoV特异性抗体的ELISA检测方法, 该方法可用于MERS病例血清抗体(IgG与IgM)的检测。

引用

页码：93 / 97

页数：5

共 14 条

[1]

Middle East respiratory syndrome coronavirus (MERS-Co V)-Republic of Korea..World Health Organization;.http://www.who.int/csr/don/03-july-2015-mers-korea/en.2015,

[2] Origin and Possible Genetic Recombination of the Middle East Respiratory Syndrome Coronavirus from the First Imported Case in China: Phylogenetics and Coalescence Analysis [J].

Wang, Yanqun ;

Liu, Di ;

Shi, Weifeng ;

Lu, Roujian ;

Wang, Wenling ;

Zhao, Yanjie ;

Deng, Yao ;

Zhou, Weimin ;

Ren, Hongguang ;

Wu, Jun ;

Wang, Yu ;

Wu, Guizhen ;

Gao, George F. ;

Tan, Wenjie .

MBIO, 2015, 6 (05)

[3] Imported case of MERS-CoV infection identified in China, May 2015: detection and lesson learned [J].

Wu, J. ;

Yi, L. ;

Zou, L. ;

Zhong, H. ;

Liang, L. ;

Song, T. ;

Song, Y. ;

Su, J. ;

Ke, C. .

EUROSURVEILLANCE, 2015, 20 (24)

[4] The Amino Acids 736-761 of the MERS-CoV Spike Protein Induce Neutralizing Antibodies: Implications for the Development of Vaccines and Antiviral Agents [J].

Yang, Yang ;

Deng, Yao ;

Wen, Bo ;

Wang, Huijuan ;

Meng, Xin ;

Lan, Jiaming ;

Gao, George F. ;

Tan, Wenjie .

VIRAL IMMUNOLOGY, 2014, 27 (10) :543-550

[5] A patient with severe respiratory failure caused by novel human coronavirus [J].

Guberina, H. ;

Witzke, O. ;

Timm, J. ;

Dittmer, U. ;

Muller, M. A. ;

Drosten, C. ;

Bonin, F. .

INFECTION, 2014, 42 (01) :203-206

[6] Investigation of Anti-Middle East Respiratory Syndrome Antibodies in Blood Donors and Slaughterhouse Workers in Jeddah and Makkah, Saudi Arabia, Fall 2012 [J].

Aburizaiza, Asad S. ;

Mattes, Frank M. ;

Azhar, Esam I. ;

Hassan, Ahmed M. ;

Memish, Ziad A. ;

Muth, Doreen ;

Meyer, Benjamin ;

Lattwein, Erik ;

Mueller, Marcel A. ;

Drosten, Christian .

JOURNAL OF INFECTIOUS DISEASES, 2014, 209 (02) :243-246

[7] Lack of MERS Coronavirus Neutralizing Antibodies in Humans, Eastern Province, Saudi Arabia [J].

Gierer, Stefanie ;

Hofmann-Winkler, Heike ;

Albuali, Waleed H. ;

Bertram, Stephanie ;

Al-Rubaish, Abdullah M. ;

Yousef, Abdullah A. ;

Al-Nafaie, Awatif N. ;

Al-Ali, Amein K. ;

Obeid, Obeid E. ;

Alkharsah, Khaled R. ;

Poehlmann, Stefan .

EMERGING INFECTIOUS DISEASES, 2013, 19 (12) :2034-2036

[8] Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study [J].

Reusken, Chantal B. E. M. ;

Haagmans, Bart L. ;

Mueller, Marcel A. ;

Gutierrez, Carlos ;

Godeke, Gert-Jan ;

Meyer, Benjamin ;

Muth, Doreen ;

Raj, V. Stalin ;

Smits-De Vries, Laura ;

Corman, Victor M. ;

Drexler, Jan-Felix ;

Smits, Saskia L. ;

El Tahir, Yasmin E. ;

De Sousa, Rita ;

van Beek, Janko ;

Nowotny, Norbert ;

van Maanen, Kees ;

Hidalgo-Hermoso, Ezequiel ;

Bosch, Berend-Jan ;

Rottier, Peter ;

Osterhaus, Albert ;

Gortazar, Christian ;

Drosten, Christian ;

Koopmans, Marion P. G. .

LANCET INFECTIOUS DISEASES, 2013, 13 (10) :859-866

[9] Seroepidemiology for MERS coronavirus using microneutralisation and pseudoparticle virus neutralisation assays reveal a high prevalence of antibody in dromedary camels in Egypt, June 2013 [J].

Perera, R. A. ;

Wang, P. ;

Gomaa, M. R. ;

El-Shesheny, R. ;

Kandeil, A. ;

Bagato, O. ;

Siu, L. Y. ;

Shehata, M. M. ;

Kayed, A. S. ;

Moatasim, Y. ;

Li, M. ;

Poon, L. L. ;

Guan, Y. ;

Webby, R. J. ;

Ali, M. A. ;

Peiris, J. S. ;

Kayali, G. .

EUROSURVEILLANCE, 2013, 18 (36) :8-14

[10] Cross-reactive antibodies in convalescent SARS patients' sera against the emerging novel human coronavirus EMC (2012) by both immunofluorescent and neutralizing antibody tests [J].

Chan, Kwok-Hung ;

Chan, Jasper Fuk-Woo ;

Tse, Herman ;

Chen, Honglin ;

Lau, Candy Choi-Yi ;

Cai, Jian-Piao ;

Tsang, Alan Ka-Lun ;

Xiao, Xincai ;

To, Kelvin Kai-Wang ;

Lau, Susanna Kar-Pui ;

Woo, Patrick Chiu-Yat ;

Zheng, Bo-Jiang ;

Wang, Ming ;

Yuen, Kwok-Yung .

JOURNAL OF INFECTION, 2013, 67 (02) :130-140

← 1 2 →