食品中乳酸菌定量检测方法研究进展

被引:14
作者
刘艳容 [1 ]
舒永红 [2 ]
杨佳玮 [2 ]
顾慧丹 [1 ]
杨瑶 [2 ]
机构
[1] 南京市产品质量监督检验院国家加工食品及食品添加剂质检中心
[2] 南京师范大学食品与制药工程学院
关键词
乳酸菌; 定量检测; 活菌计数; PMA-qPCR; 数字PCR;
D O I
10.13386/j.issn1002-0306.2020.12.059
中图分类号
TS207.4 [食品的微生物检验];
学科分类号
摘要
目前,乳酸菌发酵剂因其益生作用在乳制品生产中广泛应用。由于乳酸菌活菌数量是重要的指标,因此对乳酸菌活菌的定量检测已然成为产品质量和功能评价的一项重要工作。本文就食品中乳酸菌的定量检测方法展开综述,详细介绍了平板菌落计数法、流式细胞术、荧光定量PCR法的检测原理、关键影响因素和研究进展,重点阐述了PMA结合荧光定量PCR法的优势和技术难点以及数字PCR法的前景和可行性,为推动食品中乳酸菌定量检测技术的进步提供了参考。
引用
收藏
页码:358 / 365
页数:8
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