人参皂甙Rg1对糖尿病肾病大鼠肾脏保护作用的机制研究

被引：21

作者：

马小芬 ^{[1
]}

谢席胜 ^{[1
]}

左川 ^{[1
]}

樊均明 ^{[1
,2
]}

机构：

[1] 四川大学华西医院肾内科

[2] 四川大学华西医院人类疾病生物治疗国家重点实验室

来源：

生物医学工程学杂志 | 2010年 / 02期

关键词：

人参皂甙Rg1; 糖尿病肾病; 转化生长因子-β1; 炎症反应;

D O I：

暂无

中图分类号：

R285 [中药药理学];

学科分类号：

1008 ;

摘要：

观察人参皂甙Rg1对糖尿病大鼠肾脏的保护作用,采用链脲佐菌素(streptozotocin,STZ)腹腔注射建立糖尿病肾病(diabetic nephropathy,DN)大鼠模型,给药8周后处死各组大鼠,行肾功和24 h尿蛋白定量检查;高碘酸-希夫(氏)染色剂染色(periodic acid-Schiff,PAS)观察肾组织的病理变化;采用免疫散射比浊法检测血C-反应蛋白(cross reaction protein,CRP)水平;免疫放射法检测血肿瘤坏死因子-α(tumor necrosis factor,TNF-α)水平;免疫组织化学法检测单核/巨噬细胞表面特异性标志抗原(ectodermal dysplasia,ED-1)的表达;行酶联免疫吸附法(en-zyme-linked i mmunosorbent assay,ELISA)和实时荧光定量PCR(RT-PCR)检测肾组织转化生长因子-β1(trans-forming growth factor-β1,TGF-β1)水平。给予人参皂甙Rg1治疗的DN大鼠肾脏病理改变明显减轻,血肌酐、24 h尿蛋白、血CRP、TNF-α、ED-1、TGF-β1较模型组明显降低(P<0.05)。因此,人参皂甙Rg1能显著地减少TGF-β1和炎症反应因子水平,改善肾脏的病理损伤。

引用

页码：342 / 347

页数：6

共 16 条

[1]

Soy Protein Intake, Cardiorenal Indices, and C-Reactive Protein in Type 2 Diabetes With Nephropathy: A longitudinal randomized clinical trial[J] . Azadbakht, Leila,Atabak, Shahnaz,Esmaillzadeh, Ahmad. &nbspDiabetes Care . 2008 (4)

[2]

Different roles for TGF-beta and VEGF in the path-ogenesis of the cardinal features of diabetic nephropathy. Ziyadeh F N. Diabetes Research and Clinical Practice . 2008

[3]

Effect of inflammation on the pathogene-sis of diabetic nephropathy. QI N L,CHENJ H. Urology and nephrology FMS . 2005

[4]

Relationship between serumacute-phase proteins and diabetic nephrology in type 2 diabetic pa-tient. HE B,HAN P,LU X K. The Journal of Clinical Endocrinology . 2003

[5] Inflammation and diabetic nephropathy [J].

Mora C. ;

Navarro J.F. .

Current Diabetes Reports, 2006, 6 (6) :463-468

[6]

Analysis of relative gene expression data using real-time quantitative PCR and the 2（-Delta Delta C（T）） method. Livak KJ, Schmittgen TD. Methods . 2001

[7]

Soy protein intake, cardiorenal indices, and C-reactive protein in type 2 diabetes with nephropathy:a longitudinal randomized clinical trial. Azadbakht L,Atabak S,Esmaillzadeh A. Diabetes Care . 2008

[8]

Differential effects of ginsenosides on NO and TNF-α production by LPS-activated N9 microglia. Wu CF,Bi XL,Yang JY,Zhan JY,Dong YX,Wang JH,Wang JMi,Zhang RW,Li X. International Immunology . 2007

[9]

Diabetes,inflammation,proinflam-matory cytokines,and diabetic nephropathy. Navarro JF,Mora C. Scientif-ic World Journal . 2006

[10]

The effect of Ginsen-oside Rg1 on the renal interstitial fibrosis of UUOrat. XIE X S,LI U H C,FAN J M,et al. Si-chuan Univ （Med Sci Edi） . 2008

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