大白菜回交导入系群体构建及其遗传分析

被引：7

作者：

汪骞 ^{[1
,2
]}

和江明 ^{[3
]}

林良斌 ^{[1
]}

庄木 ^{[2
]}

王艳 ^{[2
]}

王晓武 ^{[2
]}

武剑 ^{[2
]}

机构：

[1] 云南农业大学农学与生物技术学院

[2] 中国农业科学院蔬菜花卉研究所

[3] 云南省农业科学院园艺作物研究所

来源：

园艺学报 | 2009年 / 36卷 / 09期

关键词：

大白菜; 遗传图谱; DH群体; BIL群体;

D O I：

10.16420/j.issn.0513-353x.2009.09.011

中图分类号：

S634.1 [大白菜];

学科分类号：

090202 ;

摘要：

利用大白菜栽培品种的DH系Z16和白菜型油菜自交系L144杂交的F1获得包括119个株系的DH群体。用相同亲本的F1与Z16进行独立回交,利用25个独立回交的BC2分别得到这些株系的DH系,选择每个BC2的4～6个DH系构建121个株系的BIL群体。进一步利用SSR和SRAP标记构建了DH群体遗传图谱,由10个连锁群组成,包括245个分子标记,总长度714cM,平均遗传图距2.9cM。再以此图谱为参照,用锚定在染色体上的97个SSR标记研究供体亲本L144的染色体片段在BIL群体中覆盖基因组比率。在BIL群体中来源于L144的基因组片段占0.84%～35.00%,平均为11.31%,接近理论遗传预期值12.50%。在25个BC的BIL株系中供体亲本L144等位位点占1.69%～27.36%,平均为11.03%。

引用

页码：1305 / 1310

页数：6

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