基于网络药理学的黄连解毒汤调节巨噬细胞炎症反应和代谢的作用机制研究

被引：32

作者：

衡霞 ^{[1
,2
]}

朱宝杰 ^{[1
,2
]}

孙莉敏 ^{[1
,2
]}

潘林梅 ^{[1
,2
]}

段金廒 ^{[1
,2
]}

张启春 ^{[1
,2
]}

朱华旭 ^{[1
,2
]}

机构：

[1] 南京中医药大学江苏省中药资源产业化过程协同创新中心

[2] 南京中医药大学江苏省方剂高技术研究重点实验室

来源：

药学学报 | 2018年 / 53卷 / 09期

关键词：

网络药理学; 黄连解毒汤; 炎症; 代谢;

D O I：

暂无

中图分类号：

R285 [中药药理学];

学科分类号：

100806 [中药药理学];

摘要：

运用网络药理学方法探究黄连解毒汤调节巨噬细胞炎症反应、糖酵解、鞘脂代谢和谷氨酰胺代谢等方面的多成分、多靶点、多通路的相互作用规律和调控网络,为创新药物研究奠定基础。通过TCMSP数据库筛选黄连解毒汤活性成分, PharmMapper数据库预测靶点蛋白, DAVID数据库进行通路注释和分析, Cytoscape 3.2.1软件构建"活性成分-靶点-通路"网络图, GENEMANIA数据库进行蛋白相互作用分析, Systems Dock Web Site数据库进行分子对接验证。预测结果表明,黄连解毒汤中共筛选出84个活性成分,作用靶点111个,其中与巨噬细胞炎症相关的靶点蛋白13个,涉及代谢通路14条;与糖酵解、鞘脂代谢、谷氨酰胺代谢相关的靶点蛋白34个,涉及代谢通路8条。炎症相关蛋白和代谢相关蛋白通过物理相关性、蛋白共表达等方式互相作用,小檗碱、黄芩苷和栀子苷与5个重要靶点均能较好结合。黄连解毒汤可能通过作用于糖酵解、鞘脂代谢和谷氨酰胺代谢的相关靶点,影响其代谢过程中的代谢产物和酶,从而调节巨噬细胞炎症反应。

引用

页码：1449 / 1457

页数：9

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