栀子苷对小鼠急性肺损伤保护机制的研究

被引：30

作者：

王丽莎

梅妹

机构：

[1] 吉林大学农学部门诊部

来源：

中国农学通报 | 2012年 / 28卷 / 23期

关键词：

栀子苷; 脂多糖; 急性肺损伤; 细胞因子; NF-κB;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

为了探究栀子苷能否对脂多糖(LPS)致小鼠急性肺损伤发挥保护作用及其潜在的作用机制。雄性Balb/c小鼠,于鼻腔滴注LPS前1h腹腔注射栀子苷(50mg/kg)或者地塞米松注射液(5mg/kg),滴注LPS24h后,采用酶联免疫吸附法(ELISA)测定小鼠支气管肺泡灌洗液(BALF)中肿瘤坏死因子-α(TNF-α)、白介素(IL)-6和IL-10的浓度。通过细胞分类计数法分析BALF中细胞总数、巨噬细胞和中性粒细胞的变化。采用蛋白印迹法(Western-blot)分析核转录因子-κB(NF-κB)信号转导通路激活的程度。腹腔注射栀子苷可降低小鼠肺干湿比(W/D值);改善肺组织病理学结构,减少肺泡出血和炎性细胞浸润且伴有髓过氧化物酶活性下降;抑制促炎性细胞因子TNF-α和IL-6分泌,促进抗炎性细胞因子IL-10生成。栀子苷通过缓减炎症反应保护LPS致小鼠急性肺损伤,其机制可能与阻止NF-κB信号转导通路的激活有关。

引用

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页码：26 / 31

页数：6

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[1]

甘草黄酮对小鼠急性肺损伤保护机制的研究 [J].

张佳莹 ;

魏苗苗 ;

初晓 ;

邓旭明 ;

张巧灵 ;

刘波 .

中国农学通报, 2012, (08) :56-62

[2]

New insight in LPS antagonist..Ianaro A;Tersigni M;D‘‘‘‘Acquisto F;.Mini Rev Med Chem.2009, 03

[3]

Luteolin attenuates the pulmonary inflammatory response involves abilities of antioxidation and inhibition of MAPK and NFκB pathways in mice with endotoxin-induced acute lung injury [J].

Kuo, Min-Yung ;

Liao, Mao-Fang ;

Chen, Fong-Lin ;

Li, Yi-Ching ;

Yang, Ming-Ling ;

Lin, Ruey-Hseng ;

Kuan, Yu-Hsiang .

FOOD AND CHEMICAL TOXICOLOGY, 2011, 49 (10) :2660-2666

[4]

Iridoid glycosides from Gardeniae Fructus for treatment of ankle sprain [J].

Chen, Quan Cheng ;

Zhang, Wei Yun ;

Youn, UiJoung ;

Kim, HongJin ;

Lee, IkSoo ;

Jung, Hyun-Ju ;

Na, MinKyun ;

Min, Byung-Sun ;

Bae, KiHwan .

PHYTOCHEMISTRY, 2009, 70 (06) :779-784

[5]

Inhibition of the MAP kinase ERK protects from lipopolysaccharide-induced lung injury [J].

Schuh, Katrin ;

Pahl, Andreas .

BIOCHEMICAL PHARMACOLOGY, 2009, 77 (12) :1827-1834

[6]

Gardenia jasminoides Ellis ethanol extract and its constituents reduce the risks of gastritis and reverse gastric lesions in rats [J].

Lee, Je-Hyuk ;

Lee, Dong-Ung ;

Jeong, Choon-Sik .

FOOD AND CHEMICAL TOXICOLOGY, 2009, 47 (06) :1127-1131

[7]

Local stimulation of α7 cholinergic receptors inhibits LPS-induced TNF-α release in the mouse lung [J].

Giebelen, Ida A. J. ;

van Westerloo, David J. ;

LaRosa, Gregory J. ;

de Vos, Alex F. ;

van der Poll, Tom .

SHOCK, 2007, 28 (06) :700-703

[8]

In vitro studies on the selective cytotoxic effect of crocetin and quercetin [J].

Jagadeeswaran, R ;

Thirunavukkarasu, C ;

Gunasekaran, P ;

Ramamurty, N ;

Sakthisekaran, D .

FITOTERAPIA, 2000, 71 (04) :395-399

[9]

A murine model of pulmonary damage induced by lipopolysaccharide via intranasal instillation [J].

Szarka, RJ ;

Wang, N ;

Gordon, L ;

Nation, PN ;

Smith, RH .

JOURNAL OF IMMUNOLOGICAL METHODS, 1997, 202 (01) :49-57