16S rRNA序列分析及其在乳酸菌分类、鉴定中的应用

被引：10

作者：

赵玉娟

牛春华

张雪

李达

张健

杨贞耐

机构：

[1] 吉林省农业科学院农产品加工中心

来源：

食品科学 | 2009年 / 30卷 / 07期

关键词：

乳酸菌; 16S rRNA; 分类; 鉴定;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

综述16SrRNA的特点及序列分析的基本方法,并详细介绍了几种在乳酸菌分类和鉴定中较常用的16SrRNA相关的分子生物学方法,如16SrRNA直接测序、寡核苷酸探针、限制性片段长度多态性分析、温度梯度凝胶电泳等,以及这些方法的研究进展。

引用

页码：299 / 303

页数：5

共 12 条

[1] 16S rRNA基因在细菌菌种鉴定中的应用 [J].

都立辉 ;

刘芳 .

乳业科学与技术, 2006, (05) :207-209

[2] 利用16S rRNA全长序列鉴定植物乳杆菌 [J].

涂宏钢 ;

李存瑞 ;

巫庆华 ;

王荫榆 ;

郭本恒 .

乳业科学与技术, 2004, (02) :57-60+54

[3] Sequencing of variable regions of the 16S rRNA gene for identification of lactic acid bacteria isolated from the intestinal microbiota of healthy salmonids [J].

Luis Balcazar, Jose ;

de Blas, Ignacio ;

Ruiz-Zarzuela, Imanol ;

Vendrell, Daniel ;

Girones, Olivia ;

Luis Muzquiz, Jose .

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 2007, 30 (02) :111-118

[4] Rapid identification of probiotic Lactobacillus species by multiplex PCR using species-specific primers based on the region extending from 16S rRNA through 23S rRNA [J].

Kwon, HS ;

Yang, EH ;

Yeon, SW ;

Kang, BH ;

Kim, TY .

FEMS MICROBIOLOGY LETTERS, 2004, 239 (02) :267-275

[5] A rapid method for identification of typical Leuconostoc species by 16S rDNA PCR-RFLP analysis [J].

Jang, JC ;

Kim, B ;

Lee, J ;

Han, HG .

JOURNAL OF MICROBIOLOGICAL METHODS, 2003, 55 (01) :295-302

[6]

16S–23S rDNA Intergenic Spacer Region Polymorphism of Lactococcus garvieae, Lactococcus raffinolactis and Lactococcus lactis as Revealed by PCR and Nucleotide Sequence Analysis[J] . Giuseppe Blaiotta,Olimpia Pepe,Gianluigi Mauriello,Francesco Villani,Rosamaria Andolfi,Giancarlo Moschetti.Systematic and Applied Microbiology . 2002 (4)

[7] Application of molecular methods for the classification and identification of lactic acid bacteria [J].

Schleifer, KH ;

Ehrmann, M ;

Beimfohr, C ;

Brockmann, E ;

Ludwig, W ;

Amann, R .

INTERNATIONAL DAIRY JOURNAL, 1995, 5 (08) :1081-1094

[8]

Use of the DNA sequence of variable regions of the 16S rRNA gene for rapid and accurate identification of bacteria in the Lactobacillus acidophilus complex .2 Kullen MJ,Sanozky-Dawes RB,Crowell DC,Klaenhammer TR. Journal of Applied Microbiology . 2000

[9]

Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species specific PCR primers .2 Walter,J,Tannock,GW,Tilsala-Timisjarvi,A,Rodtung,S,Loach,DM,Munro,K,Alatossava,T. Appl Environ Microbiol . 2000

[10]

Charaeterization and Identifecation of Lactic acidBacteria in "morcilla de Burgos" .2 Santos EM,Jaime I,Rovira J,etal. Int J Food Microbiol . 2005

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