壳聚糖和益生菌对暗纹东方鲀抗病力和免疫功能的影响(英文)

被引：22

作者：

华雪铭 ^{[1
]}

周洪琪 ^{[1
]}

张冬青 ^{[2
]}

李宁丽 ^{[2
]}

机构：

[1] 上海水产大学省部共建水产种质资源发掘与利用教育部重点实验室

[2] 上海交通大学医学院

来源：

水产学报 | 2007年 / 04期

基金：

上海市自然科学基金;

关键词：

暗纹东方鲀; 壳聚糖; 益生菌; 抗病能力; 免疫功能;

D O I：

暂无

中图分类号：

S943 [各种鱼的病害、敌害及其防治];

学科分类号：

摘要：

为了解壳聚糖和益生菌刺激和增强免疫应答的可能作用,本试验在基础饲料中添加0.2%、0.5%、1.0%的壳聚糖,0.1%、0.2%、0.4%的益生菌,甘露聚糖与益生菌混合物,壳聚糖与益生菌的混合物,在室内水泥池中喂养暗纹东方鲀(3.15±0.05)g 2个月,根据抗菌活力筛选出免疫增强饲料组(0.2%壳聚糖,0.1%、益生菌,甘露聚糖与益生菌混合物,壳聚糖与益生菌混合物)测定免疫功能。结果表明,甘露聚糖与益生菌混合物仅提高头肾T淋巴细胞转化,壳聚糖与益生菌混合物显著增强头肾T、B淋巴细胞转化和脾脏B淋巴细胞体外培养时分泌的IgM。筛选出的四种免疫增强饲料均能使头肾B淋巴细胞体外培养时分泌的IgM显著增加。无论是脾脏B淋巴细胞还是头肾B淋巴细胞,体外培养时所分泌的IFN-α都不同程度地受抑制。

引用

页码：478 / 486

页数：9

共 40 条

[31]

Molecular and functional analysis of an interferon genefromthe zebrafish,Danio rerio. Altmann S M,Mellon M T,Distel D L,et al. Journal of Virology . 2003

[32]

In vitroinduction of interferon-grama fromCtenopharyngodon idellussleucocytes byphytoagglutinin. Shao J Z,Xiang L X. Oceanologia et Limnologia Sinica . 2001

[33]

Immunocytochemical detection of Ig-positive cells inblood,lymphoid organs andthe gut associatedlymphoidtissue of the turbot(Scophthalmus maximus). Fournier-betz V,Quentel C,Lamour F. FishShellf Immunol . 2000

[34]

Influence ofimmunostimulant,chitosan on immune response ofhealthy and cortisol-treated rohu(Labeo rohita). Sahoo P K,Mukherjee S C. JAquac Trop . 1999

[35]

Dietary intake of immunostimulants by rainbow trout affects non-specific immunity and protection against furunculosis. Siwicki A K,et al. Veterinary Immunology and Immunopathology . 1994

[36]

Effect of multiporous microspheres derived from chitin and partially deacetylated chitin on the activation of mouse peritoneal macrophages. Nishimura K,Nishimura S,et al. Vaccine . 1987

[37]

The effect ofnatural immunomodulators appliedinimmersion on non-specific immune responses in Russiansturgen(AcipenserguelgenstaedtiBrandt). Kolman H,Siwicki A K,Kolman R. Arch Pol Fish . 1998

[38]

Humoral immune factors in the paddlefish,Polyodonspathula. Legler D W,Weinheimer P F,Acton R T,et al. Comparative Biochemistry and Physiology . 1971

[39]

Studies onsubunit components of immunoglobulin Mfroma bonyfish,the chumsalmon(Oncorynchus keta). Kobayashi K,Hara A,Takano K,et al. MoleculImmunol . 1982

[40]

Stimulation of cytokine production in mice using deacetylated chitin. Keiko Nishimura Chiaki Ishihara Shigeki Ukei Seiichi Tokura and Ichiro Azuma. Vaccine . 1986

← 1 2 3 4 →