甘蔗转HC-Pro基因的研究

被引：4

作者：

郭莺 ^{[1
]}

阮妙鸿 ^{[2
]}

吴杨 ^{[3
]}

刘佳 ^{[4
]}

杨川毓 ^{[4
]}

张木清 ^{[4
]}

机构：

[1] 福建省亚热带植物研究所、福建省亚热带植物生理生化重点公共实验室

[2] 福建省农业厅

[3] 井冈山大学生命科学学院

[4] 农业部甘蔗生理生态与遗传改良重点开放实验室

来源：

热带作物学报 | 2010年 / 06期

关键词：

RNA沉默; RNA沉默抑制子; HC-Pro; 共转化; PMI;

D O I：

暂无

中图分类号：

S566.1 [甘蔗];

学科分类号：

0901 ;

摘要：

通过基因枪(PDS-1000He)轰击法将构建好的多功能蛋白HC-Pro的植物表达载体pHCFL与带PMI筛选标记基因的pZMLR14植物表达载体共转化导入易感花叶病甘蔗品种福农95-1702的胚性愈伤组织中,转化的愈伤经甘露糖筛选后获得抗性转化甘蔗幼苗。经PCR检测、氯酚红显色、RT-PCR检测和DNA点杂交分析获得转HC-Pro的转基因植株,转化率为0.8%(HC-Pro基因)、1.7%(PMI基因)和0.4%(HC-Pro基因+PMI基因)。花叶病毒接种实验结果显示,未转基因植株的发病率为75%,而转基因植株均系统性发病,推测由于HC-Pro的大量表达抑制了甘蔗体内自身的RNA沉默机制而使病情加重。其它农艺性状调查结果显示,转基因与未转基因植株之间不存在显著差异,表明转HC-Pro和PMI基因对甘蔗的生长或其他农艺性状没有负面影响。

引用

页码：965 / 971

页数：7

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