Transgenically expressed viral RNA silencing suppressors interfere with microRNA methylation in Arabidopsis

被引：85

作者：

Yu, Bin

Chapman, Elisabeth J.

Yang, Zhiyong

Carrington, James C.

Chen, Xuemei ^{[1
]}

机构：

[1] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA

[2] Univ Calif Riverside, Inst Integrat Genome Biol, Riverside, CA 92521 USA

[3] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA

[4] Oregon State Univ, Ctr Genome Res & Biocomp, Corvallis, OR 97331 USA

来源：

FEBS LETTERS | 2006年 / 580卷 / 13期

关键词：

viral RNA silencing suppressor; methylation; microRNA/microRNA*; HEN1;

D O I：

10.1016/j.febslet.2006.04.063

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

HEN1-dependent methylation of the 3'-terminal nucleotide is a crucial step in plant microRNA (miRNA) biogenesis. Here we report that several viral RNA silencing suppressors (P1/HC-Pro, p21 and p19) inhibit miRNA methylation. These suppressors have distinct effects on different miRNAs. We also show that miRNA* is methylated in vivo in a suppressor-sensitive manner, suggesting that the viral proteins interfere with miRNA/miRNA* duplexes. p19 and p21 bind both methylated and unmethylated miRNA/miRNA* duplexes in vivo. These findings suggest miRNA/miRNA* as the in vivo substrates for the HEN1 miRNA methyltransferase and raise intriguing possibilities regarding the cellular location of miRNA methylation. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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收藏

页码：3117 / 3120

页数：4

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