碳水化合物、脂肪对翘嘴红鲌PEPCK基因表达的影响

被引：20

作者：

俞菊华

戈贤平

唐永凯

刘波

机构：

[1] 中国水产科学研究院淡水渔业研究中心农业部水生动物遗传育种和养殖生物学重点开放实验室

来源：

水产学报 | 2007年 / 03期

关键词：

翘嘴红鲌; 磷酸烯醇式丙酮酸羧激酶; 碳水化合物; 脂肪; 实时定量RT-PCR;

D O I：

暂无

中图分类号：

S917.4 [水产动物学];

学科分类号：

摘要：

使用实时定量RT-PCR测定了摄食无糖(脂肪9.13%,蛋白63.38%)、高糖(糖23.93%,脂肪9.94%,蛋白40.53%)、低脂(中糖)(脂肪9.92%,糖14.45%,蛋白50.14%)、高脂(脂肪19.93%,糖14.98%,蛋白40.88%)饲料的翘嘴红鲌在摄食后0、3、6、12、24hPEPCK的表达水平。结果显示,饲料中糖含量一致的情况下,高脂组和低脂组翘嘴红鲌PEPCK的表达差异不显著;脂肪水平一致时,高糖(23.93%)和中糖(14.45%)饲料组翘嘴红鲌PEPCK的表达,除了在0、3h时高糖组显著高外,6、12、24h没显著差异,但12、24h高糖组PEPCK相对较低,分别是中糖组的75%和65%;无糖组PEPCK的表达量除了在12h和其他各组一致(均较低)外,在其他时间均明显高,特别是摄食后24h其表达量为其他组同期的2.5～4.7倍。由此可见,当饲料中至少含14.45%糖但又低于23.93%时,饲料中的营养成分对PEPCK的表达没显著影响,而无糖饲料PEPCK的表达明显较高,和无糖饲料会引起较强的糖异生作用一致。

引用

页码：369 / 373

页数：5

共 13 条

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