紫外-常压室温等离子体复合诱变高产纤维素酶真菌

被引：24

作者：

李豪 ^{[1
]}

白光剑 ^{[1
]}

吴静 ^{[1
]}

杨海泉 ^{[2
]}

邹伟 ^{[1
]}

机构：

[1] 四川轻化工大学生物工程学院

[2] 江南大学生物工程学院

来源：

食品与发酵工业 | 2019年 / 45卷 / 15期

关键词：

枝孢菌; 纤维素酶; 紫外诱变; 常压室温等离子体诱变;

D O I：

10.13995/j.cnki.11-1802/ts.020368

中图分类号：

Q933 [微生物遗传学];

学科分类号：

071007 [遗传学];

摘要：

该研究旨在通过复合诱变技术提高真菌产纤维素酶能力。以实验室前期菌株枝孢菌(Cladosporium)B03为出发菌株,经过紫外诱变和常压室温等离子体(atmospheric and room temperature plasma,ARTP)诱变处理,通过刚果红染色初筛和酶活复筛,最终得到产酶能力提高的突变菌株AY-42。较原始菌株相比,羟甲基纤维素(carboxy methyl cellulose,CMC)酶活提高36. 14%,为(582. 14±2. 32) U/mL,滤纸酶(filter paper activity,FPA)酶活提高97. 03%,为(92. 27±0. 23) U/mL。并且产酶能力能稳定遗传。该研究通过诱变技术提高枝孢菌B03的产酶能力,为菌株B03应用于纤维素酶生产奠定基础。

引用

页码：81 / 86

页数：6

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