紫花苜蓿MsPOD基因的克隆及对拟南芥的转化

被引：12

作者：

张怡 ^{[1
,2
]}

康俊梅 ^{[2
]}

杨青川 ^{[2
]}

晁跃辉 ^{[2
]}

张铁军 ^{[2
]}

金洪 ^{[1
]}

机构：

[1] 内蒙古农业大学生态环境学院

[2] 中国农业科学院北京畜牧兽医研究所

来源：

中国草地学报 | 2013年 / 35卷 / 03期

关键词：

紫花苜蓿; 过氧化物酶; 拟南芥; 转化;

D O I：

暂无

中图分类号：

S541 [多年生豆科牧草];

学科分类号：

摘要：

利用RT-PCR技术从紫花苜蓿中克隆出过氧化物酶基因,命名为MsPOD,并通过DNA重组技术将其连接到载体pBI121上,成功构建植物表达载体pBI-MsPOD;将表达载体转化到感受态农杆菌株GV3101中,利用花序浸泡法获得具有卡纳抗性的转基因拟南芥植株。对其中3个再生植株进行PCR检测表明,目的基因已经成功整合到拟南芥基因组中,通过RT-PCR和GUS染色检测分析,初步证明目的基因在拟南芥中成功表达。

引用

页码：6 / 11

页数：6

共 18 条

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