中国李Nubiana叶柄离体培养再生植株

被引：1

作者：

孙源蔚

张陆阳

姚延兴

李国怀

机构：

[1] 教育部园艺植物生物学重点实验室华中农业大学园艺林学学院

来源：

果树学报 | 2010年 / 27卷 / 05期

关键词：

中国李; Nubiana; 叶柄; 离体培养;

D O I：

10.13925/j.cnki.gsxb.2010.05.008

中图分类号：

S662.3 [李];

学科分类号：

090201 ;

摘要：

以中国李Nubiana试管苗幼嫩叶片上的叶柄为试材,研究了基本培养基、激素种类配比、暗培养时间、培养基中琼脂浓度等对叶柄再生的影响,建立了其叶柄再生体系。结果表明,中国李Nubiana叶柄再生不定芽最佳培养基为WPM+TDZ 0.5 mg.L-1+蔗糖30 g.L-1+琼脂6 g.L-1,不定芽再生率可达到35.0%;暗培养为叶柄不定芽再生的必要条件,暗培养14 d后转入光下培养,能促进叶柄产生不定芽。中国李Nubiana叶柄再生表现出明显的极性,不定芽一般出现在叶柄的近轴端。

引用

页码：708 / 712+857 +857

页数：6

共 18 条

[11]

The effect of total inorganic nitrogen and the balance between its ionic forms on adventitious bud formation and callus growth of ‘W?gierka Zwyk?a’ plum ( Prunus domestica L.)[J] . Barbara Nowak,Kazimierz Miczyński,Ludwik Hudy.Acta Physiologiae Plantarum . 2007 (5)

[12] In vitro shoot regeneration from leaves of sweet cherry (Prunus avium) 'Lapins' and 'Sweetheart' [J].

Bhagwat, B ;

Lane, WD .

PLANT CELL TISSUE AND ORGAN CULTURE, 2004, 78 (02) :173-181

[13]

Sugar Uptake and Utilisation during Adventitious Bud Differentiation on in Vitro Leaf Explants of ‘Wegierka Zwyk?a’ Plum ( Prunus Domestica )[J] . Barbara Nowak,Kazimierz Miczyński,Ludwik Hudy.Plant Cell, Tissue and Organ Culture . 2004 (3)

[14]

Plant regeneration from leaves of sweet and sour cherry cultivars[J] . Scientia Horticulturae . 2002 (3)

[15]

Assessment of factors affecting adventitious shoot regeneration from in vitro cultured leaves of apricot[J] . Olaya Pérez-Tornero,José Egea,Alicia Vanoostende,Lorenzo Burgos.Plant Science . 2000 (1)

[16]

Plant regeneration from cotyledons of Prunus persica, Prunus domestica , and Prunus cerasus[J] . Seth Mante,Ralph Scorza,John M. Cordts.Plant Cell, Tissue and Organ Culture . 1989 (1)

[17]

Adventitions shoot formation on excised leaves of in vitro grown shoots of apple cultivars[J] . Francesca Fasolo,Richard H. Zimmerman,Ingrid Fordham.Plant Cell, Tissue and Organ Culture . 1989 (2)

[18] PLANT-REGENERATION FROM APPLE SEEDLING EXPLANTS AND CALLUS-CULTURES [J].

LIU, JR ;

SINK, KC ;

DENNIS, FG .

PLANT CELL TISSUE AND ORGAN CULTURE, 1983, 2 (04) :293-304

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