颞肌贴敷术联合自体骨髓干细胞动员治疗大鼠脑缺血

被引：18

作者：

张圣旭

步星耀

刘猛

李志营

张峰

机构：

[1] 河南省人民医院神经外科

来源：

中华临床医师杂志(电子版) | 2009年 / 3卷 / 05期

关键词：

脑缺血; 骨髓祖代细胞; 造血干细胞动员; 颞肌贴敷术;

D O I：

暂无

中图分类号：

R741 [神经病学];

学科分类号：

100204 [神经病学];

摘要：

目的探讨颞肌贴敷术联合自体骨髓干细胞动员治疗脑缺血的疗效及机制。方法选取健康成年雄性SD大鼠,线栓法制作脑缺血模型,随机分为对照组、颞肌贴敷术组、自体骨髓干细胞动员组、颞肌贴敷术联合自体骨髓干细胞动员组。采用神经功能缺损评分检测大鼠神经系统功能,病理和免疫组织化学检测组织缺血、BrdU和Ⅷ因子抗原,TUNEL法检测细胞凋亡。结果颞肌贴敷术联合自体骨髓干细胞动员组神经功能缺损评分明显低于颞肌贴敷术组和自体骨髓干细胞动员组(P<0.01),后两组神经功能缺损评分均明显低于对照组(P<0.05);组织病理显示颞肌贴敷术联合自体骨髓干细胞动员组缺血坏死面积、细胞凋亡数较颞肌贴敷术组和自体骨髓干细胞动员组明显减少(P<0.05),后两组细胞凋亡数均明显低于对照组(P<0.05);颞肌贴敷术联合自体骨髓干细胞动员组BrdU阳性细胞数、微血管数较颞肌贴敷术组和自体骨髓干细胞动员组均明显增多(P<0.05),后两组BrdU阳性细胞数、微血管数均较对照组显著增多(P<0.05)。结论颞肌贴敷术联合自体干细胞动员可通过减少梗死面积,促进血管生成,改善微循环,减少缺血灶周围细胞凋亡,增进神经的再生与修复,改善脑功能。

引用

页码：771 / 779

页数：9

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