脓毒症大鼠肠黏膜免疫屏障功能的变化

被引：3

作者：

张萌

蒋龙元

周天恩

杨正飞

温立强

常建星

机构：

[1] 中山大学附属第二医院急诊科

来源：

中华急诊医学杂志 | 2010年 / 03期

基金：

广东省自然科学基金;

关键词：

脓毒症; 黏膜免疫屏障; 防御素5; 肠三叶因子; 盲肠结扎穿孔术;

D O I：

暂无

中图分类号：

R459.7 [急症、急救处理];

学科分类号：

100218 ;

摘要：

目的研究脓毒症对大鼠肠黏膜免疫屏障功能的影响。方法 60只SD大鼠随机（随机数字法）分为对照组（n=15）和脓毒症组（n=45）,采用盲肠结扎穿孔术（CLP）建立脓毒症模型。模型建立后3 h、6 h和12 h留取回肠黏膜和全血标本。分别进行肠黏膜形态学观察、肠防御素5（RD-5）及肠三叶因子3（TFF3）mRNA表达水平检测、肠黏膜淋巴细胞凋亡分析,以及外周血中肠源性细菌DNA定性检测。结果 CLP所致脓毒症导致大鼠回肠黏膜明显损害,主要表现为上皮脱落、固有层分离、毛细血管出血和溃疡形成;脓毒症组模型建立后3 h即出现RD-5和TFF3 mRNA表达显著性减少（与正常组比较,P<0.05）,且6 h和12 h组进行下降（与3 h组比较,P<0.05）,肠黏膜淋巴细胞凋亡数亦显著增加（P<0.05）;同时,脓毒症组全血肠源性细菌DNA扩增全部阳性。结论脓毒症时大鼠肠黏膜免疫屏障功能显著减退,且随脓毒症的发展而进行性恶化。

引用

页码：264 / 268

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