姜黄素对脂多糖激活的小胶质细胞iNOS表达的抑制及抗氧化作用

被引：25

作者：

杨开艳

顾建兰

殷冬梅

沈勤

机构：

[1] 南通大学医学院生物化学教研室

来源：

中国生物化学与分子生物学报 | 2007年 / 11期

关键词：

小胶质细胞; 姜黄素; 诱导型一氧化氮合酶; 氧化应激;

D O I：

10.13865/j.cnki.cjbmb.2007.11.002

中图分类号：

R285.5 [中药实验药理];

学科分类号：

1008 ;

摘要：

用姜黄素预处理小胶质细胞株BV,1h后加用脂多糖(200ng/ml)进行刺激,通过MTT检测细胞活性;硝酸还原酶法检测细胞上清液中一氧化氮(NO)的含量;Western印迹、免疫细胞化学染色检测细胞活化后形态及诱导型一氧化氮合酶(iNOS)蛋白的表达;瞬时转染和荧光素酶报告基因鉴定iNOS和NF-κB基因表达活性;SOD和GSH-Px检测姜黄素的抗氧化能力.结果证明,脂多糖可促使小胶质细胞活化,使iNOS和NF-κB基因表达活性显著增强;iNOS蛋白表达明显上调,NO释放增多;细胞内SOD和GSH-Px活性明显下降.而姜黄素(10μmol/L)可以显著抑制活化后小胶质细胞NO的产生、iNOS蛋白的表达及iNOS-Luc、NF-κB-Luc的表达活性,其机制可能通过NF-κB的信号转导途径抑制iNOS的表达.姜黄素可通过提高细胞内SOD和GSH-Px的活性发挥抗氧化作用.

引用

页码：938 / 945

页数：8

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