用PEG法把外源基因导入甘蓝型油菜原生质体再生转基因植株

被引：14

作者：

程振东

卫志明

许智宏

机构：

[1] 中国科学院上海植物生理研究所植物分子遗传国家重点实验室,中国科学院上海植物生理研究所植物分子遗传国家重点实验室,中国科学院上海植物生理研究所植物分子遗传国家重点实验室上海,上海,上海

来源：

实验生物学报 | 1994年 / 03期

关键词：

甘蓝型油菜; 原生质体; PEG转化; 瞬间表达; 转基因植株;

D O I：

暂无

中图分类号：

S565.4 [油菜籽（芸薹）];

学科分类号：

0901 ;

摘要：

通过PEG处理把外源基因导入甘蓝型油菜原生质体。转化介质中二价阳离子的种类和浓度、携带DNA及PEG溶液的pH值都会影响基因导入效率。以潮霉素抗性和卡那霉素抗性作标记,均成功地筛选到了抗性愈伤组织。相对转化频率分别为1.3%和0.2%。前者明显高于后者。把抗性愈伤组织转到分化培养基上,分化出芽。诱导生根后移栽到土壤中,生长状况良好。酶活性测定和Southern blotting分析表明外源基因已插入到植物细胞基因组中。该遗传转化系统存在的主要问题是抗性愈伤组织分化频率较低。本文对其原因作了初步分析。

引用

页码：341 / 351

页数：11

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