大鼠全脑缺血/再灌注后TLR3 mRNA与IL-6 mRNA的表达及其相关性

被引:2
作者
万玉杰
崔桂云
沈霞
花放
陈伟
机构
[1] 徐州医学院附属医院神经内科
关键词
脑缺血/再灌注损伤; Toll样受体3; 白细胞介素-6; 海马; 大鼠;
D O I
暂无
中图分类号
R743.3 [急性脑血管疾病(中风)];
学科分类号
摘要
目的 观察大鼠全脑缺血/再灌注(I/R)后海马Toll样受体3(TLR3)mRNA与白细胞介素-6(IL-6)mRNA的表达并分析其相关性,探讨TLR3在大鼠脑I/R损伤中的作用。方法 将SD大鼠随机分为假手术组(sham组)和I/R组,采用Pulsinelli-Brierley 4动脉阻断方法制作大鼠全脑I/R损伤模型,在全脑缺血15 min后,分别再灌注6、12、24、48、72、96 h,采用RT-PCR方法检测不同时间点海马TLR3 mRNA与IL-6 mRNA的表达情况并做相关性分析。结果 与sham组相比,大鼠全脑I/R 12 h,海马TLR3 mRNA与IL-6 mRNA表达均开始增加,72 h达到高峰,96 h下降,仍高于sham组(P<0.01),6h时与sham组比较差异无统计学意义(P>0.05)。TLR3 mRNA与IL-6 mRNA的表达呈正相关(r=0.959,P<0.01)。结论 大鼠脑I/R后TLR3 mRNA表达上调,可能通过促使炎性细胞因子的分泌介导脑I/R炎性损伤。
引用
收藏
页码:145 / 148
页数:4
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