钙离子在介导SA诱导白桦悬浮细胞三萜合成途径中的作用

被引：22

作者：

王艳

尹静

马泓思

詹亚光

苏欣

梁甜

张梦岩

机构：

[1] 东北林业大学生命科学学院,林木遗传育种与生物技术国家重点实验室

来源：

北京林业大学学报 | 2014年 / 02期

基金：

黑龙江省自然科学基金;

关键词：

白桦; 三萜物质; 关键基因; 钙调蛋白;

D O I：

暂无

中图分类号：

S792.153 [白桦];

学科分类号：

090708 [林木遗传育种学];

摘要：

为探讨Ca2+作为信号分子在水杨酸(SA)诱导白桦悬浮细胞三萜合成中的作用,本文用SA、Ca2+及Ca2+阻断剂对白桦悬浮细胞进行处理,采用紫外分光光度法和高效液相色谱法测定细胞中总三萜及其组分的积累量;同时利用荧光定量PCR方法检测了白桦细胞中三萜合成途径关键酶基因(FPS、SS、SE、BPW、BPY、CAS)和钙调蛋白基因表达变化。结果显示:水杨酸可诱导白桦悬浮细胞三萜物质(总三萜、齐墩果酸、白桦脂醇、白桦脂酸)的合成及相关基因的上调表达,且Ca2+的加入使得这种诱导效应更为显著,暗示白桦细胞中钙信号分子介导了水杨酸诱导三萜物质的合成。而对SA与各种Ca2+阻断剂互作处理结果显示,Ca2+抑制剂的加入抑制了SA对细胞中三萜合成的诱导效果,表明Ca2+作为信号分子介导了SA诱导白桦悬浮细胞三萜物质的合成。

引用

页码：51 / 58

页数：8

共 22 条

[11]

诱导子组合对白桦三萜合成调控及MeJA抑制性消减文库分析 [D].

任春林 .

东北林业大学,

2012

[12]

The inhibitory effect of Ca<Superscript>2+</Superscript> on the flavonoid production of <Emphasis Type="Italic">Tetrastigma hemsleyanum</Emphasis> suspension cells induced by metal elicitors[J] Xin Peng;Jun-Yao He In Vitro Cellular & Developmental Biology - Plant 2013,

[13]

Synthesis of betulinic acid derivatives as entry inhibitors against HIV-1 and bevirimat-resistant HIV-1 variants[J] Zhao Dang;Keduo Qian;Phong Ho;Lei Zhu;Kuo-Hsiung Lee;Li Huang;Chin-Ho Chen Bioorganic & Medicinal Chemistry Letters 2012,

[14]

Distribution and expression characteristics of triterpenoids and OSC genes in white birch ( Betula platyphylla suk.)[J] Jing Yin;Chun-Lin Ren;Ya-Guang Zhan;Chun-Xiao Li;Jia-Lei Xiao;Wei Qiu;Xin-Yu Li;Hong-Mei Peng Molecular Biology Reports 2012,

[15]

A Common Structural Basis for pH- and Calmodulin-mediated Regulation in Plant Glutamate Decarboxylase[J] Heinz Gut;Paola Dominici;Stefania Pilati;Alessandra Astegno;Maxim V. Petoukhov;Dmitri I. Svergun;Markus G. Grütter;Guido Capitani Journal of Molecular Biology 2009,

[16]

Immunotoxicity and allergenic properties of betulin-containing birch bark dry extract[J] L. P. Kovalenko;V. V. Balakshin;G. A. Presnova;A. N. Chistyakov;E. V. Shipaeva;S. V. Alekseeva;A. D. Durnev Pharmaceutical Chemistry Journal 2007,

[17]

Elicitor signal transduction leading to production of plant secondary metabolites[J] Jian Zhao;Lawrence C. Davis;Robert Verpoorte Biotechnology Advances 2005,

[18]

Selective cytotoxicity of betulinic acid on tumor cell lines; but not on normal cells[J] Valentina Zuco;Rosanna Supino;Sabina C Righetti;Loredana Cleris;Edoardo Marchesi;Carlo Gambacorti-Passerini;Franca Formelli Cancer Letters 2002,

[19]

Effect of salicylic acid; methyl jasmonate; ethephon and cantharidin on anthraquinone production by Rubia cordifolia callus cultures transformed with the rolB and rolC genes[J] V.P Bulgakov;G.K Tchernoded;N.P Mischenko;M.V Khodakovskaya;V.P Glazunov;S.V Radchenko;E.V Zvereva;S.A Fedoreyev;Yu.N Zhuravlev Journal of Biotechnology 2002,

[20]

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2 ?ΔΔ C T Method[J] Kenneth J. Livak;Thomas D. Schmittgen Methods 2001,

← 1 2 3 →