微透析-HPLC-ECD联用技术用于香附四物汤对大鼠脑内5-HIAA动态变化的评价(英文)

被引：19

作者：

刘培

段金廒

刘睿

郭建明

唐于平

机构：

[1] 南京中医药大学江苏省方剂研究重点实验室

来源：

中华中医药杂志 | 2011年 / 26卷 / 05期

关键词：

5-HIAA; 微透析; HPLC-ECD; 原发性痛经; 香附四物汤;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

原发性痛经被认为是与性激素紊乱伴随月经前的孕酮减少有关。下丘脑的单胺和其代谢产物可能参与了这个病理过程。用微透析结合HPLC-ECD快速鉴定正常和原发性痛经模型大鼠下丘脑中的5-HIAA。微透析样品中5-HIAA的检测限和定量限分别是1.5ng/mL和5.0ng/mL。校准曲线在10-250ng/mL的浓度范围内为线性。痛经模型大鼠的5-HIAA的含量和正常对照组相比有显著的增加,表明原发性痛经导致5-HIAA的增加。经过香附四物汤的治疗,5-HIAA的含量降低,显示香附四物汤通过降低下丘脑的5-羟色胺的代谢率从而减轻原发性痛经的疼痛综合症。

引用

页码：902 / 907

页数：6

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