锌指核酸酶在基因组靶向修饰中的应用

被引：10

作者：

王令

张存芳

张智英

机构：

[1] 西北农林科技大学动物科技学院

来源：

中国生物化学与分子生物学报 | 2009年 / 25卷 / 07期

关键词：

锌指核酸酶; 同源重组; 基因组; 靶向修饰; 基因治疗;

D O I：

10.13865/j.cnki.cjbmb.2009.07.001

中图分类号：

Q783.1 [DNA分子的切割酶];

学科分类号：

071007 ; 0836 ; 090102 ;

摘要：

同源重组和逆转录病毒介导转基因法是目前基因组修饰中常用的两种主要方法.由于这些传统方法效率低,特异性差等缺点,制约了其在研究中的应用.锌指核酸酶(zinc finger nuclease,ZFN)是一种人工合成酶,含有锌指蛋白DNA结合域和非特异性核酸酶FokI结构域.ZFN在对基因组的靶向修饰时,表现出高度特异性和高效性.最新研究结果显示,锌指核酸酶在哺乳动物细胞和斑马鱼基因组靶向敲除的效率高达20%.这一技术的出现,将给基因组靶向修饰的研究和应用领域带来革命,特别是在基因治疗人类疾病方面有巨大的潜力和广阔的前景.

引用

页码：585 / 589

页数：5

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