HPLC-CAD一测多评法同时测定黄芪中6种成分含量

被引：41

作者：

姚静 ^{[1
]}

孙欣光 ^{[1
]}

董蓉 ^{[1
]}

谢建宏 ^{[1
]}

王玉龙 ^{[2
]}

杨晓宁 ^{[1
]}

机构：

[1] 北京振东光明药物研究院有限公司

[2] 山西振东道地药材开发有限公司

来源：

药学学报 | 2021年 / 56卷 / 02期

基金：

国家重点研发计划;

关键词：

HPLC-CAD; 一测多评; 相对校正因子; 黄芪; 含量测定; 主成分分析;

D O I：

暂无

中图分类号：

R284.1 [化学分析与鉴定];

学科分类号：

100804 [中药化学];

摘要：

建立高效液相串联电雾式检测器（HPLC-CAD）同时测定黄芪药材中黄芪皂苷Ⅰ、黄芪皂苷Ⅱ、黄芪甲苷、毛蕊异黄酮葡萄糖苷、芒柄花素和7,2'-二羟基-3',4'-二甲氧基异黄烷的一测多评方法。采用Agilent SB-C18 （150 mm×4.6 mm, 3.5μm）色谱柱, 0.05%甲酸水-0.05%甲酸乙腈为流动相,梯度洗脱,流速1.0 mL·min-1,柱温35℃,进样量为20μL; CAD雾化室温度50℃。建立内参物黄芪皂苷Ⅱ与其他成分的相对校正因子,并通过相对校正因子计算其含量。同时采用外标法验证所建立的一测多评法的合理性、可行性和重复性。结果表明:各色谱峰分离度较高,黄芪皂苷Ⅰ、黄芪皂苷Ⅱ、黄芪甲苷、毛蕊异黄酮葡萄糖苷、芒柄花素和7,2'-二羟基-3',4'-二甲氧基异黄烷分别在0.113～2.250、0.012～0.240、0.004～0.080、0.065～1.300、0.005～0.100和0.007～0.150 mg·mL-1内线性关系良好; 20批黄芪药材中黄芪皂苷Ⅰ、黄芪皂苷Ⅱ、黄芪甲苷、毛蕊异黄酮葡萄糖苷、芒柄花素、7,2'-二羟基-3',4'-二甲氧基异黄烷的含量分别为0.306～0.922、0.053～0.183、0.015～0.092、0.069～0.823、0～0.098和0.020～0.107 mg·g-1;内参物黄芪皂苷Ⅱ与黄芪皂苷Ⅰ、黄芪甲苷、毛蕊异黄酮葡萄糖苷、芒柄花素和7,2'-二羟基-3',4'-二甲氧基异黄烷的相对校正因子分别为0.561、0.835、0.299、0.796和0.799;一测多评法含量测定结果与外标法测定结果无显著性差异。建立的HPLC-CAD一测多评法简便、准确,可用于黄芪中6种化学成分的含量测定,研究结果为黄芪药材的质量评价提供了科学依据。

引用

页码：557 / 564

页数：8

共 24 条

[1]

Simultaneous Determination of Six Chromones in Saposhnikoviae Radix via Quantitative Analysis of Multicomponents by Single Marker [J].

Zhang, Jinhua ;

Chen, Luxiao ;

Qiu, Junna ;

Zhang, Yu ;

Wang, Lu ;

Jiang, Lijuan ;

Jiang, Yanyan ;

Liu, Bin .

JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY, 2020, 2020

[2]

Quality assessment of Astragali Radix from different production areas by simultaneous determination of thirteen major compounds using tandem UV/charged aerosol detector.[J].Cong-En Zhang;Li-Juan Liang;Xiao-Hong Yu;Hao Wu;Peng-fei Tu;Zhi-Jie Ma;Kui-Jun Zhao.Journal of Pharmaceutical and Biomedical Analysis.2019,

[3]

Simultaneous determination of seven phenylethanoid glycosides in Cistanches Herba by a single marker using a new calculation of relative correction factor [J].

Dong, Yanhong ;

Guo, Qi ;

Liu, Jingjing ;

Ma, Xueqin .

JOURNAL OF SEPARATION SCIENCE, 2018, 41 (09) :1913-1922

[4]

Comparative analysis of multiple representative components in the herb pair Astragali Radix-Curcumae Rhizoma and its single herbs by UPLC-QQQ-MS.[J].Gang Yin;Xiaolan Cheng;Weiwei Tao;Yu Dong;Yong Bian;Wenhua Zang;Decai Tang.Journal of Pharmaceutical and Biomedical Analysis.2018,

[5]

A novel combination of four flavonoids derived from Astragali Radix relieves the symptoms of cyclophosphamide-induced anemic rats [J].

Zhang, Li ;

Gong, Amy G. W. ;

Riaz, Kashif ;

Deng, Jun Y. ;

Ho, Chih M. ;

Lin, Huang Q. ;

Dong, Tina T. X. ;

Lee, Yi-Kuen ;

Tsim, Karl W. K. .

FEBS OPEN BIO, 2017, 7 (03) :318-323

[6]

Astragaloside IV from Astragalus membranaceus ameliorates renal interstitial fibrosis by inhibiting inflammation via TLR4/NF-кB in vivo and in vitro.[J].Xiangjun Zhou;Xinbo Sun;Xiaoxin Gong;Yong Yang;Congbo Chen;Guang Shan;Qisheng Yao.International Immunopharmacology.2017,

[7]

Triterpenoids and Flavonoids from the Leaves of Astragalus membranaceus and Their Inhibitory Effects on Nitric Oxide Production [J].

Wang, Zhi-Bin ;

Zhai, Ya-Dong ;

Ma, Zhen-Ping ;

Yang, Chun-Juan ;

Pan, Rong ;

Yu, Jia-Li ;

Wang, Qiu-Hong ;

Yang, Bing-You ;

Kuang, Hai-Xue .

CHEMISTRY & BIODIVERSITY, 2015, 12 (10) :1575-1584

[8]

A systematic study on the influencing parameters and improvement of quantitative analysis of multi-component with single marker method using notoginseng as research subject.[J].Chao-Qun Wang;Xiu-Hong Jia;Shu Zhu;Katsuko Komatsu;Xuan Wang;Shao-Qing Cai.Talanta.2015,

[9]

Review of the Botanical Characteristics, Phytochemistry, and Pharmacology of Astragalus membranaceus (Huangqi) [J].