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基因枪介导转化水稻花药愈伤获得抗白叶枯病转基因植株

被引：10
作者：
万丙良
张献龙
机构：
[1] 华中农业大学作物遗传改良国家重点实验室!武汉
来源：
华中农业大学学报 | 2001年 / 01期
关键词：
水稻; 花药愈伤; 基因枪; 转基因;
D O I：
10.13300/j.cnki.hnlkxb.2001.01.001
中图分类号：
S511.035.3 [];
学科分类号：
摘要：
以质粒pBI12 1作外源DNA ,对基因枪介导转化水稻花药愈伤的有关参数进行了优化 ,优化值分别为射程 9cm ,枪膛氦气压力 110 0psi,真空度 36 0 0～ 3733Pa ,轰击次数 2次。轰击前后愈伤经 0 .4mol/L甘露醇处理可提高转化效率。在优化条件下将Xa2 1基因导入籼型光敏核不育水稻W945 1S ,获得了单倍体植株 ,经人工染色体加倍得倒二倍体转基因植株。白叶枯病菌接种鉴定结果表明其抗性有明显提高
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页码：1 / 6
页数：6
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