适配体芯片技术及其应用

被引：3

作者：

甘龙杰

兰小鹏

机构：

[1] 南京军区福州总医院全军医学检验研究所

来源：

临床检验杂志 | 2009年 / 27卷 / 06期

关键词：

适配体; 芯片; 靶物质; 检测;

D O I：

10.13602/j.cnki.jcls.2009.06.029

中图分类号：

R450 [];

学科分类号：

摘要：

<正>适配体(aptamers)是通过指数级富集配体的系统进化技术(systematicevolution of ligands by exponential enrichment,SELEX)筛选而获得的某种单链DNA或RNA分子,这些分子能够折叠成特定的三维空间结构与其所针对的各种靶物质特异地结合,而不是依赖特异性的碱基排列。适配体芯片又称适配体微阵列,是继基因芯片、蛋白质芯片之后发展起来的一种新芯片。

引用

页码：425 / 426+433 +433

页数：3

共 6 条

[1] Chip-based detection of hepatitis C virus using RNA aptamers that specifically bind to HCV core antigen
Lee, Seram
Kim, Young Sook
Jo, Minjung
Jin, Moonsoo
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[2] Optimization of aptamer microarray technology for multiple protein targets
Cho, EJ
Collett, JR
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Ellington, AD
[J]. ANALYTICA CHIMICA ACTA, 2006, 564 (01) : 82 - 90
[3] Production and processing of aptamer microarrays
Collett, JR
Cho, EJ
Ellington, AD
[J]. METHODS, 2005, 37 (01) : 4 - 15
[4] Aptamer-based biosensor arrays for detection and quantification of biological macromolecules
McCauley, TG
Hamaguchi, N
Stanton, M
[J]. ANALYTICAL BIOCHEMISTRY, 2003, 319 (02) : 244 - 250
[5] The use of biosensor and microarray techniques in the rapid detection and identification of salmonellae .2 Goldschmidt MC. J AOAC Int . 2006
[6] Aptamer-antibody on-chip sandwich immunoassay for detection of CRP in spiked serum .2 Pultar J,Sauer U,Domnanich P,Preininger C. Biosens Bioelectron . 2009

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