依达拉奉对大鼠缺血/再灌注心肌细胞凋亡的影响

被引：5

作者：

吕云波

曾秋棠

机构：

[1] 华中科技大学同济医学院附属协和医院心内科

来源：

实用医学杂志 | 2008年 / 16期

关键词：

心肌缺血; 心肌再灌注损伤; 细胞凋亡; 依达拉秦;

D O I：

暂无

中图分类号：

R96 [药理学];

学科分类号：

100602 ; 100706 ;

摘要：

目的:观察依达拉奉对心肌缺血/再灌注后心肌细胞凋亡的影响。方法:选择30只健康SD大鼠,采用结扎冠状动脉左前降支后再通的方法复制心肌缺血再灌注的动物模型,随机分为假手术组(n=10)、缺血/再灌注(I/R)组(n=10)和药物(依达拉秦)组(n=10)。检测各组3h后心肌组织的超氧化物歧化酶(SOD)和丙二醛(MDA)的含量。并用免疫组化法测定局部凋亡相关因子Bcl-2、Fas的表达,比较各组间差异。结果:与假手术组比较,I/R组中反映氧化损伤程度的MDA明显升高(P<0.01),抗氧化酶SOD则明显减少(P<0.01),Fas含量升高(P<0.01),Bcl-2含量明显减少(P<0.01);药物组较I/R组MDA含量明显减少(P<0.01),SOD活性显著增加(P<0.01),Fas含量明显降低(P<0.05),Bcl-2含量明显增加(P<0.01)。结论:依达拉奉具有抗心肌缺血再灌注损伤作用,其机制可能是通过调节Bcl-2和Fas介导的细胞凋亡而实现。

引用

页码：2764 / 2766

页数：3

共 10 条

[1] 匹那地尔对未成熟兔心肌保护的实验研究(英文) [J].

林茹 ;

朱雄凯 ;

张泽伟 ;

康曼丽 ;

夏强 .

ChineseMedicalJournal, 2001, (11)

[2] An antioxidant treatment potentially protects myocardial energy metabolism by regulating uncoupling protein 2 expression in a chronic β-adrenergic stimulation rat model [J].

Ishizawa, Makoto ;

Mizushige, Katsufumi ;

Noma, Takahisa ;

Namba, Tsunetatsu ;

Guo, Peng ;

Murakami, Kazushi ;

Tsuji, Teppei ;

Miyatake, Akira ;

Ohmori, Koji ;

Kohno, Masakazu .

LIFE SCIENCES, 2006, 78 (25) :2974-2982

[3] The mitochondrial death pathway and cardiac myocyte apoptosis [J].

Crow, MT ;

Mani, K ;

Nam, YJ ;

Kitsis, RN .

CIRCULATION RESEARCH, 2004, 95 (10) :957-970

[4] Effects of edaravone on reperfusion injury in patients with acute myocardial infarction [J].

Tsujita, K ;

Shimomura, H ;

Kawano, H ;

Hokamaki, J ;

Fukuda, M ;

Yamashita, T ;

Hida, S ;

Nakamura, Y ;

Nagayoshi, Y ;

Sakamoto, T ;

Yoshimura, M ;

Arai, H ;

Ogawa, H .

AMERICAN JOURNAL OF CARDIOLOGY, 2004, 94 (04) :481-484

[5]

Edaravone, a newly developed radical scavenger, protects against ischemia-reperfusioninjury of the small intestine in rats[J] . Naoya Tomatsuri,Norimasa Yoshida,Tomohisa Takagi,Kazuhiro Katada,Yutaka Isozaki,Eiko Imamoto,Kazuhiko Uchiyama,Satoshi Kokura,Hiroshi Ichikawa,Yuji Naito,Takeshi Okanoue,Toshikazu Yoshikawa.International Journal of Molecular Medicine . 2004 (1)

[6] Prevention of ischemic heart failure by exercise in spontaneously diabetic BB Wor rats subjected to insulin withdrawal [J].

Villanueva, DS ;

Poirier, P ;

Standley, PR ;

Broderick, TL .

METABOLISM-CLINICAL AND EXPERIMENTAL, 2003, 52 (06) :791-797

[7]

Efficacy of Edaravone, a Free Radical Scavenger, on Left Ventricular Function and Structure in Diabetes Mellitus[J] . Tetsuya Hayashi,Tatsuhiko Mori,Koichi Sohmiya,Yoshikatsu Okada,Sakiko Inamoto,Nobuaki Okuda,Hiroshi Mori,Yasushi Kitaura.Journal of Cardiovascular Pharmacology . 2003 (6)

[8]

Vitamin D target proteins: Function and regulation[J] . S.Christakos,F.Barletta,M.Huening,P.Dhawan,Y.Liu,A.Porta,X.Peng.J. Cell. Biochem. . 2003 (2)

[9] Humic acid induces apoptosis in human endothelial cells [J].

Hseu, YC ;

Huang, HW ;

Wang, SY ;

Chen, HY ;

Lu, FJ ;

Gau, RJ ;

Yang, HL .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 2002, 182 (01) :34-43

[10]

Oxidative,stress and neutrophil aciation-the two key stone of ischemiareperfusion injury. Kaminski K A,Bonda T A,Korecki J,el al. International Journal of Cardiology . 2002

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