温针灸对兔膝骨性关节炎模型软骨细胞中c-Jun氨基末端激酶信号通路的影响

被引：25

作者：

武永利

刘娣

马晓秀

李春

机构：

[1] 宁夏医科大学总医院中医骨伤科

来源：

中国中西医结合杂志 | 2017年 / 37卷 / 11期

关键词：

温针灸; 膝骨关节炎; 基质金属蛋白酶; c-Jun氨基末端激酶信号通路;

D O I：

暂无

中图分类号：

R245 [针灸学、针灸疗法]; R-332 [医用实验动物学];

学科分类号：

100402 [劳动卫生与环境卫生学]; 100517 [针灸学];

摘要：

目的观察温针灸治疗兔膝骨性关节炎(KOA)模型软骨中c-Jun氨基末端激酶(JNK)信号通路及基质金属蛋白酶(MMP)-1、MMP-13的表达,为温针灸治疗KOA提供现代医学理论支持。方法 40只新西兰大耳兔随机选择30只采用右膝关节伸直位石膏固定制备KOA模型,另10只为正常对照组。固定6周后行X线检查确定造模成功后随机分为温针灸组、双氯芬酸钠组、模型组,每组10只。温针灸组选取足三里、鹤顶、内膝眼穴给予温针灸治疗,每天1次,15min/次,治疗2周;双氯芬酸钠组按15mg/kg灌胃,每天1次,治疗2周;模型组及正常对照组不予任何干预,正常饲养。采用免疫组织化学法、RT-PCR、免疫蛋白印迹法(Western Blot)检测软骨中JNK1、JNK2、MMP-1、MMP-13的表达。结果与正常对照组比较,模型组关节软骨中MMP-1、MMP-13、JNK1、JNK2mRNA及蛋白表达升高(P<0.05),且温针灸组、双氯芬酸钠组关节软骨中MMP-1、MMP-13、JNK1、JNK2mRNA及蛋白表达较模型组降低(P<0.05),同时温针灸组MMP-1、MMP-13mRNA蛋白表达及JNK1、JNK2 mRNA表达较双氯芬酸钠组降低更为明显(P<0.05)。结论温针灸可抑制KOA软骨细胞中JNK1、JNK2、MMP-1、MMP-13的表达,温针灸治疗KOA可能是通过激活JNK信号通路,抑制MMP-1、MMP-13的表达而达到治疗作用。

引用

页码：1372 / 1377

页数：6

共 13 条

[1]

Garlic-derived compound S-allylmercaptocysteine inhibits cell growth and induces apoptosis via the JNK and p38 pathways in human colorectal carcinoma cells [J].

Zhang, Yan ;

Li, Hong-Yan ;

Zhang, Zhi-Hua ;

Bian, Hong-Lei ;

Lin, Gui .

ONCOLOGY LETTERS, 2014, 8 (06) :2591-2596

[2]

The Role of Inflammatory and Anti-Inflammatory Cytokines in the Pathogenesis of Osteoarthritis.[J].Piotr Wojdasiewicz;?ukasz A. Poniatowski;Dariusz Szukiewicz;Chuanju Liu.Mediators of Inflammation.2014,

[3]

MicroRNA-27b Regulates the Expression of Matrix Metalloproteinase 13 in Human Osteoarthritis Chondrocytes [J].

Akhtar, Nahid ;

Rasheed, Zafar ;

Ramamurthy, Sangeetha ;

Anbazhagan, Arivarasu N. ;

Voss, Frank R. ;

Haqqi, Tariq M. .

ARTHRITIS AND RHEUMATISM, 2010, 62 (05) :1361-1371

[4]

PGE2 inhibits MMP expression by suppressing MKK4–JNK MAP kinase–c‐JUN pathway via EP4 in human articular chondrocytes.[J].KoheiNishitani;HiromuIto;TerukoHiramitsu;RyosukeTsutsumi;ShimeiTanida;ToshiyukiKitaori;HiroyukiYoshitomi;MasahikoKobayashi;TakashiNakamura.J. Cell. Biochem..2010, 2

[5]

Osteoarthritic cartilage chondrocytes alter subchondral bone osteoblast differentiation via MAPK signalling pathway involving ERK1/2.[J].Indira Prasadam;Thor Friis;Wei Shi;Stijn van Gennip;Ross Crawford;Yin Xiao.Bone.2009, 1

[6]

Signal transduction pathways involving p38 MAPK, JNK, NFκB and AP-1 influences the response of chondrocytes cultured in agarose constructs to IL-1β and dynamic compression [J].

Chowdhury, T. T. ;

Salter, D. M. ;

Bader, D. L. ;

Lee, D. A. .

INFLAMMATION RESEARCH, 2008, 57 (07) :306-313

[7]

Induction of matrix metalloproteinase-13 gene expression by TNF-α is mediated by MAP kinases, AP-1, and NF-κB transcription factors in articular chondrocytes [J].

Liacini, A ;

Sylvester, J ;

Li, WQ ;

Huang, WS ;

Dehnade, F ;

Ahmad, M ;

Zafarullah, M .

EXPERIMENTAL CELL RESEARCH, 2003, 288 (01) :208-217

[8]

Interleukin-17 signal transduction pathways implicated in inducing matrix metalloproteinase-3; -13 and aggrecanase-1 genes in articular chondrocytes.[J].Judith Sylvester;Abdelhamid Liacini;Wen Qing Li;Muhammad Zafarullah.Cellular Signalling.2003, 4

[9]

EXPERIMENTAL OSTEOARTHRITIS IN THE RABBIT - COMPARISON OF DIFFERENT PERIODS OF REPEATED IMMOBILIZATION [J].

VIDEMAN, T .

ACTA ORTHOPAEDICA SCANDINAVICA, 1982, 53 (03) :339-347

[10]

炎性反应在骨关节炎软骨退变中的作用 [J].

郑洁 ;

王瑞辉 ;

寇久社 .

基础医学与临床, 2014, 34 (08) :1146-1149

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