猪肉中15种喹诺酮类药物残留的高效液相色谱-串联质谱法研究

被引：3

作者：

张志刚 ^{[1
]}

张洁 ^{[2
]}

刘宗保 ^{[3
]}

机构：

[1] 肉食品安全生产技术国家重点实验室,食品安全与营养协同创新中心,厦门银祥集团有限公司

[2] 中国科学院城市环境研究所

[3] 华南理工大学轻工与食品学院

来源：

广东农业科学 | 2013年 / 40卷 / 24期

关键词：

猪肉; 喹诺酮; 液相色谱; 质谱; 残留;

D O I：

10.16768/j.issn.1004-874x.2013.24.004

中图分类号：

O657.63 [质谱分析]; TS251.7 [产品标准与检验];

学科分类号：

070302 ; 081704 ; 083203 ;

摘要：

利用高效液相色谱-电喷雾串联质谱建立了猪肉中15种喹诺酮类药物残留的同时、快速分析方法。猪肉样品中残留药物用1%乙酸-乙腈均质提取,浓缩后用0.1%甲酸-乙腈定容,正己烷脱脂,用Kinetex C18色谱柱（100mm×2.1 mm,2.6μm）分离,以0.1%甲酸-乙腈作为流动相梯度洗脱,采用基于正离子扫描的MRM监测模式,用保留时间和离子对（母离子和碎片离子）定性,响应值高的碎片离子作为定量离子,基质匹配外标法定量。结果表明,15种喹诺酮类药物在1～500μg/kg范围内具有良好的线性关系,定量限在1～8μg/kg之间。在3个添加水平下,加标回收率为60.1%91.4%,相对标准偏差为0.42%6.28%。该方法操作简便、灵敏度高、特异性好,可用于猪肉中喹诺酮类药物残留的高通量检测。

引用

页码：91 / 95

页数：5

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