高效液相色谱法测定水产品中9种氟喹诺酮类药物残留

被引:17
作者
惠芸华
张晓玲
金高娃
于慧娟
机构
[1] 中国水产科学研究院东海水产研究所农业部水产品质量监督检验测试中心
关键词
高效液相色谱法; 水产品; 氟喹诺酮;
D O I
暂无
中图分类号
S948 [水产药物学、药理学];
学科分类号
090403 [农药学];
摘要
建立了固相萃取-反相高效液相色谱法,同时分析了测定水产品种中9种氟喹诺酮类药物残留的方法。样品用酸化乙腈提取,正己烷去脂,C18固相萃取柱净化后,用高效液相色谱法分离,荧光检测器检测,外标法定量。诺氟沙星、环丙沙星、培氟沙星、二氟沙星和恩诺沙星检出限为1.0μg/kg,单诺沙星为0.2μg/kg,沙拉沙星为2.0μg/kg,氧氟沙星为5.0μg/kg,麻保沙星为50.0μg/kg。各组分平均回收率在75%98%之间,相对标准偏差为0.5%9.0%。本方法灵敏度高,成本低,通用性强。
引用
收藏
页码:204 / 210
页数:7
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