血脉通颗粒对动脉粥样硬化小鼠外周血单核细胞TLR4、IL-6、TNF-α的影响

被引：8

作者：

丁志祥

钱高潮

张琪

机构：

[1] 南京中医药大学附属常州市中医医院

来源：

山东医药 | 2013年 / 53卷 / 44期

关键词：

动脉粥样硬化; 血脉通颗粒; Toll样受体4; 白细胞介素6; 肿瘤坏死因子α; 小鼠;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

1008 ;

摘要：

目的观察血脉通颗粒对动脉粥样硬化(AS)小鼠外周血单核细胞Toll样受体4(TLR4)表达及血浆白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)水平的影响。方法选择apo E基因敲除(apo E-/-)小鼠,采用高脂饮食法建立AS模型;造模成功后随机分为血脉通组和对照组,每组11只。血脉通组给予血脉通颗粒3.808 mg/(g·d)灌胃,对照组给予相同剂量生理盐水灌胃。6周后处死,眼眶静脉丛采血。采用RT-PCR法检测小鼠外周血单核细胞TLR4 mRNA,流式细胞术测算单核细胞表面TLR4平均荧光强度,ELISA法检测小鼠血浆IL-6和TNF-α。结果血脉通组外周血单核细胞中TLR4 mRNA的相对表达量为0.22±0.07,TLR4荧光强度为25.45±8.30,血浆IL-6、TNF-α水平分别为(10.86±4.41)、(36.94±9.46)pg/mL;对照组分别为34.04±7.31、0.31±0.09及(18.24±8.21)、(52.82±13.78)pg/mL;两组比较,P均<0.05。小鼠血浆IL-6、TNF-α水平与单核细胞表面TLR4的表达均呈正相关(r=4.86、0.509,P均<0.05)。结论血脉通颗粒可降低AS小鼠单核细胞TLR4表达和血浆IL-6、TNF-α水平,这可能是其抑制血管炎症、治疗AS的机制之一。

引用

页码：4 / 6+112 +112

页数：4

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