基质辅助激光解吸电离飞行时间质谱技术在临床微生物鉴定中的应用及价值

被引：12

作者：

顾兵 ^{[1
]}

李永军 ^{[2
]}

机构：

[1] 南京医科大学第一附属医院检验学部

[2] 梅里埃诊断产品上海有限公司

来源：

临床检验杂志 | 2013年 / 31卷 / 11期

关键词：

基质辅助激光解吸电离飞行时间; 质谱; 回报时间; 微生物; 感染性疾病;

D O I：

10.13602/j.cnki.jcls.2013.11.007

中图分类号：

R446.5 [微生物学检验];

学科分类号：

100208 ;

摘要：

20世纪90年代末,基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization time of flight mass spectrometry,MALDI-TOF MS)技术开始用于病原菌鉴定,目前已逐步用于临床微生物检验工作,对临床感染性疾病的快速诊断意义重大。本文阐述MALDI-TOF MS技术鉴定微生物的原理和流程、在临床微生物鉴定中的应用和对微生物检验结果回报时间(TAT)改进的作用。

引用

页码：814 / 817

页数：4

共 24 条

[1] 用MALDI-TOF MS技术快速鉴定临床分离棒状杆菌属细菌 [J].

徐志江 ;

周宏伟 ;

孙谦 ;

胡燕燕 ;

陈功祥 ;

张嵘 .

临床检验杂志, 2012, 30 (09) :668-670

[2] 基质辅助激光解吸电离飞行时间质谱在临床微生物领域的应用进展 [J].

颜英俊 ;

汤一苇 .

国际检验医学杂志, 2011, 32 (17) :1969-1972

[3] Improvement of MALDI-TOF MS profiling for the differentiation of species within the Acinetobacter calcoaceticus-Acinetobacter baumannii complex [J].

Sedo, Ondrej ;

Nemec, Alexandr ;

Krizova, Lenka ;

Kacalova, Magdalena ;

Zdrahal, Zbynek .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 2013, 36 (08) :572-578

[4] Impact of rapid microbial identification directly from positive blood cultures using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry on patient management [J].

Martiny, D. ;

Debaugnies, F. ;

Gateff, D. ;

Gerard, M. ;

Aoun, M. ;

Martin, C. ;

Konopnicki, D. ;

Loizidou, A. ;

Georgala, A. ;

Hainaut, M. ;

Chantrenne, M. ;

Dediste, A. ;

Vandenberg, O. ;

Van Praet, S. .

CLINICAL MICROBIOLOGY AND INFECTION, 2013, 19 (12) :1173-1173

[5]

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry in Clinical Microbiology[J] . Robin Patel.Clinical Infectious Diseases . 2013 (4)

[6] Evaluation of MALDI-TOF mass spectrometry for the identification of medically-important yeasts in the clinical laboratories of Dijon and Lille hospitals [J].

Sendid, Boualem ;

Ducoroy, Patrick ;

Francois, Nadine ;

Lucchi, Geraldine ;

Spinali, Sebastien ;

Vagner, Odile ;

Damiens, Sebastien ;

Bonnin, Alain ;

Poulain, Daniel ;

Dalle, Frederic .

MEDICAL MYCOLOGY, 2013, 51 (01) :25-32

[7]

Novel Approach for Differentiating Shigella Species and Escherichia coli by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry[J] . Prasanna D. Khot,Mark A. Fisher.Journal of Clinical Microbiology . 2013 (11)

[8]

Performance of MALDI-ToF MS for species identification of Burkholderia cepacia complex clinical isolates[J] . Lorena Cristina Corrêa Fehlberg,Lucas Henrique Sales Andrade,Diego Magno Assis,Rosana Helena Vicente Pereira,Ana Cristina Gales,Elizabeth Andrade Marques.Diagnostic Microbiology & Infectious Disease . 2013

[9]

Prospective Evaluation of a Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System in a Hospital Clinical Microbiology Laboratory for Identification of Bacteria and Yeasts: a Bench-by-Bench Study for Assessing the Impact on Time to Identification and Cost-Effectiveness[J] . K. E. Tan,B. C. Ellis,R. Lee,P. D. Stamper,S. X. Zhang,K. C. Carroll.Journal of Clinical Microbiology . 2012 (10)

[10] MALDI-TOF MS in microbiological diagnostics-identification of microorganisms and beyond (mini review) [J].

Wieser, Andreas ;

Schneider, Lukas ;

Jung, Jette ;

Schubert, Soeren .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2012, 93 (03) :965-974

← 1 2 3 →