毛白杨纤维素合酶基因家族部分成员的克隆及表达

被引:6
作者
陈亚娟
王宏芝
李瑞芬
张中保
崔莉洁
魏建华
机构
[1] 北京市农林科学院北京农业生物技术研究中心
关键词
毛白杨; 纤维素合酶; GeXP; 基因表达;
D O I
暂无
中图分类号
S792.11 [杨];
学科分类号
0829 ; 0907 ;
摘要
从毛白杨中分离7个纤维素合酶基因,分别为PtoCesA4,PtoCesA5,PtoCesA7,PtoCesA8,PtoCesA13,PtoCesA17和PtoCesA18,通过与已公布的毛果杨全基因序列及拟南芥和水稻基因组中纤维素合酶基因序列的同源性比对分析,结合目前纤维素合成机制的研究进展,预测毛白杨中上述不同纤维素合酶的功能。利用GenomeLabTMGeXP遗传分析系统分析毛白杨中纤维素合酶基因在不同组织中的转录表达水平,结果表明PtoCesA4,PtoCesA7,PtoCesA8,PtoCesA17和PtoCesA18在木质部高丰度表达,推测这些基因可能参与毛白杨次生细胞壁的形成,为毛白杨次生细胞壁中纤维素的合成调控提供参考依据。
引用
收藏
页码:70 / 75
页数:6
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