南方红豆杉bHLH基因克隆与序列分析

被引：10

作者：

周华 ^{[1
]}

朱祺 ^{[1
]}

杨艳芳 ^{[2
]}

刘洪伟 ^{[2
]}

余发新 ^{[1
]}

邱德有 ^{[2
]}

机构：

[1] 江西省观赏植物遗传改良重点实验室,江西省科学院生物资源研究所

[2] 林木遗传育种国家重点实验室,中国林业科学研究院林业研究所,林木遗传育种国家重点实验室

来源：

植物研究 | 2015年 / 35卷 / 01期

关键词：

红豆杉属; b HLH; 基因克隆; 序列分析; 紫杉醇;

D O I：

暂无

中图分类号：

Q943.2 [植物基因工程]; S791.49 [红豆杉、紫杉];

学科分类号：

摘要：

以南方红豆杉为试材,利用RT-PCR方法从南方红豆杉c DNA中克隆了一个b HLH转录因子——Tc MYC,Gen Bank注册号为KC878013。通过生物信息学分析,发现该基因全长1 959 bp,编码一个650个氨基酸残基的亲水蛋白;Tc MYC相对分子量为71.4 k D,等电点p I为4.87。推测所克隆Tc MYC基因编码的蛋白定位于细胞核中,含有一个螺旋—环—螺旋结构,与东北红豆杉、葡萄和烟草的MYC基因编码的蛋白序列一致性分别为98%、45%和44%,进化树分析表明同科属植物的MYC转录因子归为一类,红豆杉的MYC与其他草本被子植物的MYC转录因子亲缘关系都较远。在茉莉酸甲酯诱导的红豆杉细胞中,Tc MYC基因表达呈现略微下降趋势,推测Tc MYC可能负向调控紫杉醇生物合成,为利用分子手段调控紫杉醇的生物合成提供了理论基础。

引用

页码：52 / 59

页数：8

共 19 条

[11]

Deep sequencing reveals transcriptome reprogramming of Taxus×media cells to the elicitation with methyl jasmonate. Sun GL,Yang YF,Xie FL,Wen JF,Wu JQ,Wilson IW,Tang Q,Liu HW,Qiu DY. PLoS One . 2013

[12]

The Taxol Supply Crisis. New NCI Policies for Handling the Large-Scale Production of Novel Natural Product Anticancer and Anti-HIV Agents. Gordon M. Cragg,Saul A. Schepartz,Matthew Suffness,Michael R. Grever. Journal of Natural Products . 1993

[13]

A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins. Murre C,McCaw P S,Baltimore D. Cell . 1989

[14]

Identification and in silico characterization of soybean trihelix-GT and bHLH transcription factors involved in stress responses. Marina Borges Osorio,Lauro Bücker-Neto,Graciela Castilhos,Andreia Carina Turchetto-Zolet,Beatriz Wiebke-Strohm,Maria Helena Bodanese-Zanettini,Márcia Margis-Pinheiro. Genet. Mol. Biol . 2012

[15]

A transcription factor with a bHLH domain regulates root hair development in rice. Ding Wona,Yu Zhiming,Tong Yanli,Huang Wei,Chen Hanmin,Wu Ping. Cell Research . 2009

[16]

Taxus metabolomics: methyl jasmonate preferentially induces production of taxoids oxygenated at C-13 in Taxus x media cell cultures[J] . Raymond E.B. Ketchum,Christopher D. Rithner,Deyou Qiu,You Sun Kim,Robert M. Williams,Rodney B. Croteau. &nbspPhytochemistry . 2003 (6)

[17] Multiple Phytochrome-Interacting bHLH Transcription Factors Repress Premature Seedling Photomorphogenesis in Darkness [J].

Leivar, Pablo ;

Monte, Elena ;

Oka, Yoshito ;

Liu, Tiffany ;

Carle, Christine ;

Castillon, Alicia ;

Huq, Enamul ;

Quail, Peter H. .

CURRENT BIOLOGY, 2008, 18 (23) :1815-1823

[18]

Isolation of WDR and bHLH genes related to flavonoid synthesis in grapevine ( Vitis vinifera L.)[J] . &nbspPlant Molecular Biology . 2010 (6)

[19]

Identification and characterization of MYB ‐b HLH ‐ WD 40 regulatory complexes controlling proanthocyanidin biosynthesis in strawberry ( F ragaria × ananassa ) fruits[J] . Jan G. Schaart,Christian Dubos,Irene Romero De La Fuente,Adèle M. M. L. Houwelingen,Ric C. H. Vos,Harry H. Jonker,Wenjia Xu,Jean‐Marc Routaboul,Lo?c Lepiniec,Arnaud G. Bovy. &nbspNew Phytol . 2012 (2)

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